UBE2E3 Mouse mAb - Anticorps primaire, specific to UBE2E3, Rabbit IgG

Cat. No.: Ab133237

GRADE & PURITY See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Souris

Reactive species

Cependant, conseil, Humain, Monkey, Souris

Isotype

Mouse IgG1

Clone Number

OTI1D12

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human UBE2E3 Antibody, Monoclonal (OTI7E8), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

UBE2E3 Mouse mAb - Anticorps primaire, specific to UBE2E3, Rabbit IgG

Synonymes

UbcM2 | UBCH9 | UBE2E3 | Ubiquitin-protein ligase E3 antibody | Ubiquitin-conjugating enzyme E2-23 kDa antibody | UB2E3_HUMAN antibody | Homologous to yeast UBC4/5 antibody | UBC E4 antibody | Ubc M2 antibody | UBC4/5 homolog antibody | Ubiquitin-conjugat

Spécifications et pureté

See COA

Mécanismes biochimiques et physiologiques

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Participates in the regulation of transepithelial sodium transport in

Espèce hôte

Souris

Spécificité

UBE2E3

Immunogène

Recombinant protein of human UBE2E3 (AA 1-207).

Contrôle positif

WB: Hep-G2, HeLa, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF7 lysates. IHC: Human colon, kidney, ovary, pancreas, thyroid, endometrium, prostate, bladder tissue. IF/ICC: HEK293E cells. Flow Cytometry: HEK293T cells.

Conjugation

Unconjugated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Méthode de purification

Protein A/G purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/100;

• IHC:

  1/150

• IF/ICC:

  1:100 ;

Cibles associées (humaines)

UBE2E3 Tbio Ubiquitin-conjugating enzyme E2 E3 (0 Activities)

Discover Target: UBE2E3

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (UBE2E3 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.