UltraBio™ Multiplex Amplification Mix - BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,2.5μL/T

Cat. No.: FP1508887

GRADE & PURITY Suitable for molecular biology, BioReagent, DNase, RNase free, PCR Reagent, for DNA and RNA applications, 2.5μL/T

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Application

Multiplex PCR, PCR

Vue d’ensemble

UltraBio™ Multiple Amplification Mix is a universal multiplex amplification reagent that can be used for constructing multiplex amplicon libraries for microbial/genetic mutation detection. It is compatible with ultra-multiplex amplification, with a primer number range of 1 to 1500, amplicon length range of 100 to 1800 bp, GC content range of 13% to 75%, and a minimum template input amount of 1 ng. 

   The reagent undergoes strict background microbe quality control to minimize false positives from non-specific amplification. 

   Rigorous functional validation ensures high stability and reproducibility, making it ideal for NGS-based multiplex targeted amplification in microbial/genetic mutation detection.

Catalog	Component	24T	96T	Storage
FP1508887	UltraBio™ Multiplex Amplification Mix	60 μL	240 μL	-20℃

Self-Supplied Materials

1. Instruments

    PCR thermal cycler, Pipettes, Magnetic stand, Microcentrifuge, Vortex mixer, Agarose gel electrophoresis system, UV gel imaging system, Agilent Bioanalyzer 2100 (or equivalent).

2. Reagents

    Agarose, 1× TAE buffer, Nucleic acid dye, DNA marker, Ampure XP beads (or equivalent), Freshly prepared 80% ethanol, Nuclease-free water.

3. Consumables

    1.5 mL microcentrifuge tubes, 0.2 mL PCR tubes, DNase/RNase-free pipette tips.

Protocol

I. Multiplex Amplification & Product Verification

1. Reagent Preparation

   • Thaw UltraBio™ Multiplex Amplification Mix on ice.

   • Vortex briefly and centrifuge before use.

2. Reaction Setup (10 μL Standard Volume)

3. Component	Volume per Reaction	Final Concentration
   UltraBio™ Multiplex Amplification mix	2.5 μL	4x
   Primer Mix	1 μL	20nM (2.5~100nM)
   Template DNA	X μL	10ng (1~100ng)
   Nuclease-free water	Up to10 μL	/
   Total	10 μL	/

Note:

• Total reaction volume can be scaled to 10–50 μL (adjust mix proportionally).

• Primer concentration and annealing temperature (T) should be optimized for each panel.

1. Mixing & Centrifugation

   • Vortex gently and centrifuge briefly to collect liquid.

2. PCR Cycling Program

*T: Annealing temperature depends on primer panel (optimize empirically).
Cycle number: Adjust based on template input (default: 25 cycles).

1. Product QC: Analyze amplification success via 2% agarose gel electrophoresis or Agilent 2100 Bioanalyzer.

II. Amplicon Purification (Ampure XP Beads)

1. Bead Equilibration

   • Warm Ampure XP beads to room temperature; vortex thoroughly.

2. Bead Binding

   • Dilute PCR products to 50 μL with nuclease-free water.

   • Add 60 μL beads (1.2× ratio), vortex, and incubate 5 min at RT.

3. Wash Steps

   • Place tubes on a magnetic stand for 3–5 min; discard supernatant.

   • Wash twice with 200 μL fresh 80% ethanol (30 sec per wash).

   • Air-dry beads for 2–3 min until matte.

4. Elution

   • Resuspend beads in 22 μL nuclease-free water; incubate 2 min at RT.

   • Transfer 20 μL supernatant to a new PCR tube.

   • Store purified products at –20°C.
     

Specifications

Spécifications et pureté

BioReagent,DNase, RNase free,PCR Reagent,Suitable for molecular biology,for DNA and RNA applications,2.5μL/T

Stabilité et stockage

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Upon receipt, it is recommended to aliquot.

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Grade

BioReagent, DNase, RNase free, for DNA and RNA applications, PCR Reagent, Suitable for molecular biology

Foire aux questions

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does DNase, RNase free mean?

DNase, RNase free indicates that the product has been processed and tested for sensitive nucleic acid and protein workflows. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.