Vancomycin (Vancomycin) ELISA Kit

Cat. No.: EJ1515208

GRADE & PURITY BioReagent

Vue d’ensemble

This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Vancomycin antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Vancomycin in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.

Specifications

Spécifications et pureté

BioReagent

Grade

BioReagent

Application

ELISA

Type d'échantillon

homogénats tissulaires, Lysat cellulaire, Plasma, Sérum, surnageants de culture cellulaire

Plage de détection

1.56ng/mL~100ng/mL

Spécificité

Detecting Vancomycin in samples, with no significant cross-reactivity to other homologs.

Detection instrument

Microplate reader

Detection wavelength

450nm

Repeatability

The intra- and inter-assay coefficients of variation (CV) are both <10%.

Detection duration

3.5 hours, not including equilibration and sample preparation time.

Stockage et expédition

Conditions de stockage de stockage

Store at 2-8°C

Expédié en

Wet ice

Stabilité et stockage

Store at 2-8℃ for 6 months.