X-34 (Amyloid-Specific Fluorescent Dye) - BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥90%(HPLC) , CAS No.215294-98-7

CAS: 215294-98-7 Cat. No.: X1444884 Formule: C₂₄H₁₈O₆ Poids moléculaire: 402.4 PubChem CID: 9930943

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥90%(HPLC)

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Staining‌, Fluorescence Analysis

Vue d’ensemble

X-34 is a lipophilic and highly fluorescent marker used to examine pathological changes in Alzheimer’s disease (AD). This cellular dye was developed as a derivative of Congo red to be more compatible with in vivo applications. By replacing the azo groups (nitrogen-to-nitrogen double bonds) in Congo red with carbon-to-carbon double bonds and having more lipophilic salicylic acid groups, X-34 has proven to be highly fluorescent and have increased membrane permeability. X-34 detects β-sheet structures and labels amyloid plaques, neurofibrillary tangles (NFTs), neuropil threads, and vascular amyloid in AD brains (1).

Product Usage Information

Reagents:

1. X-34:Create a 5 mM stock solution of X-34 (200X) using the X-34 diluent. Create a 25 µM staining solution by further diluting the 5 mM stock solution using the X-34 diluent.

Note: X-34 diluent (40% EtOH, pH 10) is the recommended solvent for staining. A slight precipitate may be present, but will not interfere with the product's performance.

2. X-34 diluent (40% EtOH, pH 10): Combine 20 mL of EtOH in 29.5 mL of dH2O. Add 1 µL of 5M NaOH dropwise until the solution reaches pH 10. Bring the final volume of X-34 diluent to 50 mL with dH2O.

3. Differentiation buffer: 50 mM NaOH, 80% EtOH

Protocol:

1. Stain slides in 25 µM X-34 staining solution for 30 min at room temperature in the dark.

2. Rinse slides three times in dH2O.

3. Incubate for 2 min in the differentiation buffer.

4. Rinse slides three times in dH2O.

5. If desired, proceed with immunostaining.

6. Mount and image.

Specifications

Spécifications et pureté

BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥90%(HPLC)

Stabilité et stockage

Store at -20℃ long term (12 months).

Conditions de stockage de stockage

Store at -20°C

Expédié en

Ice chest + Ice pads

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Grade

Biological Stain, BioReagent, pour l'analyse par fluorescence, pour la microscopie

Pureté

≥90%(HPLC)

Noms et identifiants

• Sourires canoniques: C1=CC(=CC=C1C=CC2=CC(=C(C=C2)O)C(=O)O)C=CC3=CC(=C(C=C3)O)C(=O)O
• IUPAC Name: 5-[(E)-2-[4-[(E)-2-(3-carboxy-4-hydroxyphenyl)ethenyl]phenyl]ethenyl]-2-hydroxybenzoic acid
• InChIKey: MCBNOAYTZBUCSX-KQQUZDAGSA-N
• INCHI: 1S/C24H18O6/c25-21-11-9-17(13-19(21)23(27)28)7-5-15-1-2-16(4-3-15)6-8-18-10-12-22(26)20(14-18)24(29)30/h1-14,25-26H,(H,27,28)(H,29,30)/b7-5+,8-6+
• Isomères SMILES: C1=CC(=CC=C1/C=C/C2=CC(=C(C=C2)O)C(=O)O)/C=C/C3=CC(=C(C=C3)O)C(=O)O
• PubChem CID: 9930943
• Poids moléculaire: 402.4

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Phenylpropanoids and polyketides
    • Classe Stilbenes

• Kingdom: Organic compounds
• Superclass: Phenylpropanoids and polyketides
• Classe: Stilbenes
• Subclass: Not available
• Intermediate Tree Nodes: Not available
• Direct Parent: Stilbenes
• Alternative Parents: Salicylic acids Benzoic acids Styrenes Benzoyl derivatives 1-hydroxy-2-unsubstituted benzenoids Vinylogous acids Carboxylic acids Organooxygen compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic homomonocyclic compounds
• Substituents: Stilbene - Salicylic acid - Salicylic acid or derivatives - Hydroxybenzoic acid - Benzoic acid or derivatives - Benzoic acid - Benzoyl - Styrene - 1-hydroxy-2-unsubstituted benzenoid - Phenol - Monocyclic benzene moiety - Benzenoid - Vinylogous acid - Carboxylic acid - Carboxylic acid derivative - Hydrocarbon derivative - Organic oxide - Organic oxygen compound - Organooxygen compound - Aromatic homomonocyclic compound
• Description: This compound belongs to the class of organic compounds known as stilbenes. These are organic compounds containing a 1,2-diphenylethylene moiety. Stilbenes (C6-C2-C6 ) are derived from the common phenylpropene (C6-C3) skeleton building block. The introduction of one or more hydroxyl groups to a phenyl ring lead to stilbenoids.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Propriétés chimiques et physiques

• Solubilité: DMSO: 2.0mg/mL