Yeast Nitrogen Base Agar - BioReagent

Cat. No.: Y1528772

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Vue d’ensemble

For classification of yeasts based on carbon source utilization

Specifications

Spécifications et pureté

BioReagent

Conditions de stockage de stockage

Room temperature

Expédié en

Normal

Grade

BioReagent

Application Protocols

Below are general, literature-based protocols for using Yeast Nitrogen Base Agar. For this SKU’s exact preparation instructions (grams per liter, pH targets), follow the product label and CoA/Spec Sheet.

• Standard YNB Agar plate preparation (general):

  1. Suspend the specified mass of YNB Agar per liter in purified water with stirring.
  2. Heat to dissolve agar completely. Adjust pH if required.
  3. Autoclave using a validated cycle (e.g., 121 °C, 15–20 minutes; adjust per volume).
  4. Cool to ~50–55 °C. Aseptically add sterile-filtered supplements (e.g., 10–20 g/L glucose for carbon source; defined amino acid drop-out mixes for selection) as needed.
  5. Pour ~20–25 mL per 90 mm plate. Allow to solidify and dry to a matte surface before use. Store plates at 2–8 °C in sealed sleeves if not used immediately (check stability under your conditions).

• Plating and incubation (general):

  • Streak or spread 10^2–10^6 cells depending on assay. Incubate inverted plates at the organism’s optimal temperature (commonly 25–30 °C for many yeasts; strain dependent) until colonies develop.

• Quality checks:

  • Include sterility controls (uninoculated plate) and positive controls (reference strain) to confirm performance. Record lot numbers for reproducibility.

Note: Times, temperatures, and supplement concentrations are literature examples; tailor to your strain and experimental goals.

Biological Roles

The product itself does not have a biological role as a molecule; rather, it serves as a defined growth environment supporting yeast physiology studies.

• Use in studying biological processes (general, literature):

  • Carbon and nitrogen metabolism: YNB formulations provide inorganic nitrogen and essential salts/vitamins, enabling controlled evaluation of how yeasts utilize different carbon sources or respond to nitrogen limitation.
  • Genetic selection: By omitting specific nutrients (e.g., uracil, leucine, histidine), researchers can select for plasmid maintenance or gene knockouts in auxotrophic strains.
  • Stress responses: Defined salt and pH conditions facilitate reproducible assays of osmotic, oxidative, or pH stress and their transcriptional consequences.
  • Morphogenesis and mating: Supplemented YNB Agar can be adapted to assess filamentation, mating type interactions, and sporulation efficiency.

• Advantages of a defined agar matrix (general):

  • Reproducibility: Reduced variability versus complex media supports quantitative comparison across experiments and labs.
  • Analytical compatibility: Lower background from undefined peptides or yeast extract benefits metabolomics, lipidomics, and secreted metabolite profiling from colony growth.

Note: Exact nutrient composition, including vitamin and trace element levels, should be obtained from the product’s CoA/Spec Sheet for experiments requiring precise mass balances.

Buffer Applications

This product is a growth medium, not a dedicated buffering system. While prepared YNB Agar exhibits a characteristic pH suitable for yeast cultivation, it is not intended to provide strong buffering capacity.

• Practical notes (general, literature):
  • If precise pH control is required for experimental design (e.g., pH-sensitive transport studies), incorporate an appropriate biological buffer (e.g., citrate, phosphate, or MES) into the medium and verify compatibility with your yeast strain and selection markers.
  • Adjust pH prior to sterilization; confirm final pH after autoclaving and cooling, as thermal processing can shift pH.

For dedicated buffer recipes and titration behavior, use standard buffer systems rather than relying on YNB Agar.

Green Alternatives

YNB Agar is water-based and generally benign compared to many solvent-intensive workflows. Environmental considerations focus on sourcing, sterilization energy, and waste handling rather than chemical substitution.

• Sustainability considerations (general):

  • Agar origin: Agar is typically derived from seaweed (renewable biomass). Confirm supplier sourcing policies if marine sustainability is a priority.
  • Energy use: Autoclaving is energy-intensive. Consolidate sterilization cycles, use validated minimum hold times, and prepare only the volume needed to reduce energy and waste.
  • Waste minimization: Prepare smaller batches to limit expired plates; use stackable plate drying methods to reduce condensation and plate loss.

• Alternatives and trade-offs (literature/general):

  • Gellan gum can substitute for agar in some applications, often at lower concentrations; however, it may require specific cation levels for optimal gelation and can alter diffusion properties.
  • Rich complex media (e.g., YPD agar) may provide faster growth but increase biochemical background for downstream analytics; YNB Agar maintains a defined composition that reduces interference.

• Packaging and disposal:

  • Choose bulk packaging sizes that match consumption to reduce plastic waste. Autoclave biological waste prior to disposal, adhering to local regulations.

No hazardous solvent replacements are relevant because preparation is strictly aqueous.

Pharmaceutical Uses

No therapeutic or clinical applications are claimed. For research use only (per Product Data).

• Relevant non-clinical/industrial contexts (general):

  • Microbiological quality control in research and development labs, including environmental isolates of yeasts and defined selection of production strains (where permitted by SOPs).
  • Strain maintenance and genetic verification of yeast hosts used in recombinant protein expression or bioprocess development (research phase).

• Formulation/excipient status:

  • Not an excipient and not intended for drug product formulation.
  • No pharmacopeial monograph is implied for this specific formulation unless otherwise stated on documentation.

• Compliance notes:

  • For regulated manufacturing or release testing, use media qualified to the appropriate compendial or cGMP standard. This SKU is BioReagent grade and intended for research applications unless your quality system deems it suitable after qualification.

Always verify internal QA requirements before deploying research-grade media in any regulated workflows.

Physical Properties

• Item-specific properties from Product Data:

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Storage Conditions: Room temperature (per Product Data).
  • Shipped In: Normal (per Product Data).
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

• Physical constants (not provided for this specific item):

  • Melting point, boiling point, density, refractive index: Not applicable/not specified for this item; refer to CoA/Spec Sheet.

• General/literature characteristics of YNB Agar (for context; actual values vary by supplier and lot):

  • Form: Free-flowing, dehydrated powder blend containing agar. Color ranges from off-white to light tan (literature).
  • Solubility: Intended to dissolve/disperse in purified water upon heating; agar requires heating to near boiling to melt and fully dissolve (literature).
  • Final medium pH: YNB-based media commonly target mildly acidic pH suitable for yeasts (e.g., ~5.4–6.2 at 25 °C; literature—formulation dependent). Users should adjust pH as needed for specific strains and supplements.
  • Osmolality/ionic strength: Determined by the salt composition of the YNB; typically suitable for Saccharomyces and other yeasts in defined conditions (literature).

Note: For precise composition, pH at 25 °C, and recommended grams per liter for this SKU, consult the product label and CoA/Specification Sheet. Do not rely on generic values when reproducing critical experiments.

Quality and Grades

• Item-specific grade: BioReagent (per Product Data).

• What BioReagent grade implies (general guidance):

  • Intended for laboratory research applications. Components and performance characteristics are suitable for biological experiments (e.g., culture of microorganisms) where controlled background and reproducibility are needed.
  • Typically emphasizes low bioburden, consistent nutrient profile, and batch-to-batch reproducibility. It is not produced under cGMP for clinical diagnostics or therapeutic manufacturing unless explicitly stated.

• Relevance to YNB Agar:

  • For defined yeast work, BioReagent grade minimizes lot variability in salts, vitamins, and agar gel strength, supporting reproducible growth curves and selection experiments.
  • UV absorbance, trace metals, and other analytical attributes may be controlled in-house but are not specified here. For any critical parameter (e.g., trace metal background for metabolomics), request the CoA/Spec Sheet or a detailed QA statement.

• Stabilizers/additives:

  • Not specified for this item; refer to CoA/Spec Sheet. No assumptions should be made about pH indicators, antibiotics, or carbon sources being included unless stated on the label.

• Documentation:

  • For performance testing (e.g., growth promotion with reference strains), sterility assurance, and recommended preparation, consult the lot-specific CoA/Spec Sheet.

Reaction and Applications

Chemical reactions are not the primary context for this product. Instead, Yeast Nitrogen Base Agar is used as a defined, solid growth medium for yeasts and related fungi in research settings.

• Typical research applications (literature/general):

  • Selection and maintenance of auxotrophic strains by omitting specific amino acids or bases from the formulation while supplying a defined carbon source (e.g., glucose, galactose, raffinose, glycerol/ethanol).
  • Phenotypic assays (growth rate, colony morphology), mating and sporulation studies (with appropriate supplements), and genetic screening in Saccharomyces and non-Saccharomyces yeasts.
  • Metabolic studies under defined nitrogen/salt/vitamin conditions to minimize media background in metabolomics or gene expression experiments.

• Practical tips (general):

  • Carbon source is typically not included in YNB Agar; add sterile carbohydrate to the desired final concentration post-autoclave when the medium has cooled to ~50–55 °C.
  • Antibiotics, amino acids, bases, and trace elements can be filter-sterilized and added post-autoclave for selective media.
  • Pour plates at consistent depth (e.g., ~20–25 mL per standard 90 mm plate) to ensure uniform colony size and comparable diffusion properties.

Note: Follow the label/CoA of this SKU for exact grams per liter and any pre-included supplements. Do not rely solely on generic recipes when reproducing critical experiments.

Reaction Conditions

Chemical reaction parameters (temperature, catalysts, yields) are not applicable. The relevant “conditions” pertain to media preparation and sterilization for microbiology.

• General preparation conditions (literature; follow your label/CoA for this SKU):

  • Reconstitution: Disperse the specified grams of YNB Agar per liter of purified water while stirring.
  • Dissolution: Heat with agitation to near boiling until the agar fully dissolves; avoid localized overheating.
  • Sterilization: Autoclave using a validated cycle (commonly 121 °C for 15–20 minutes for 1 L; adjust for volume and autoclave performance). Do not over-autoclave to prevent degradation of heat-labile components.
  • Post-sterilization: Cool to ~50–55 °C. Aseptically add sterile-filtered supplements (e.g., carbon sources, amino acids, antibiotics) as required by the experiment. Mix gently to avoid bubble formation.
  • Plate pouring: Dispense into sterile plates at consistent volumes. Allow to solidify and dry with lids ajar under aseptic conditions to achieve the desired surface moisture.

• Performance checks:

  • Optional growth-promotion testing using reference yeast strains can verify lot performance under your specific supplement/pH conditions.

Note: Exact mass per liter, target pH, and supplement lists are not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

• GHS classification and hazard statements (item-specific):

  • Signal Word: Not specified for this item; refer to SDS.
  • H-Statements: Not specified for this item; refer to SDS.
  • GHS Classification: Not specified for this item; refer to SDS.
  • Pictograms: Not specified for this item; refer to SDS.

• General laboratory safety (applicable to dehydrated microbiological media; literature):

  • Avoid creating dust; fine powders may cause mechanical irritation to the respiratory tract and eyes. Use local exhaust/HEPA fume hood when weighing.
  • Personal protective equipment: laboratory coat, safety glasses or goggles, and appropriate gloves (e.g., nitrile). Wash hands after handling.
  • Incompatibilities: Store away from strong oxidizing agents and moisture. Hygroscopic components can cake and compromise performance.
  • First aid (overview; defer to SDS): If inhaled, move to fresh air; for eye contact, rinse cautiously with water for several minutes; if skin contact occurs, wash with soap and water; if ingested, rinse mouth—seek medical advice if symptoms persist.

• Microbiological practice notes (research use):

  • Sterilize prepared medium by autoclaving as appropriate. Use aseptic technique to avoid contamination.
  • Dispose of used plates/medium by autoclaving prior to discarding, following institutional biosafety and local regulations.

Always consult the product-specific SDS for authoritative safety information before use.

Solvent Selection

This product is a dehydrated microbiological medium intended to be reconstituted in purified water; organic solvent selection is not applicable.

• Practical guidance (general, literature):

  • Use deionized/distilled water (Type II or better) for reconstitution. Hard water or high ionic content can alter gel strength and growth performance.
  • Agar requires heating to near boiling to fully dissolve; mix vigorously while heating to avoid clumping. Avoid scorching.
  • After dissolution, adjust pH if required for your organism/assay, then sterilize by autoclaving (e.g., 121 °C). Cool to ~50–55 °C before pouring plates to prevent condensation and component degradation.

• Miscibility profile: Not applicable beyond aqueous preparation. Do not prepare with alcohols or other organics unless a specific protocol calls for sterile supplements added after autoclaving.

For solvent considerations related to extraction/analytics performed on cultures grown on this medium, select solvents appropriate to your analytes; the medium itself is water-based when prepared.

Storage and Reconstitution

• Item-specific storage/shipping (from Product Data):

  • Storage Conditions (dehydrated powder): Room temperature.
  • Shipped In: Normal.

• Reconstitution (general guidance; follow the product label/CoA for exact details):

  • Water quality: Use purified water. Add the specified grams of YNB Agar per liter while stirring.
  • Dissolution: Heat to fully dissolve agar; avoid scorching. Skim foam if necessary to ensure clear pours.
  • Sterilization: Autoclave using a validated cycle appropriate for your volume. Do not over-sterilize to avoid nutrient degradation.
  • Post-autoclave handling: Cool to ~50–55 °C before adding sterile supplements (carbon source, amino acids, antibiotics). Mix gently and pour plates aseptically.

• Prepared medium/plate storage (general):

  • Store poured plates in sealed sleeves or wrapped stacks to minimize dehydration and contamination. Typical storage 2–8 °C, protected from light; shelf life depends on formulation and supplements. Monitor for drying, contamination, or color change.

• Freeze–thaw: Not applicable for dehydrated powder; do not freeze prepared agar plates as it disrupts gel integrity.

For lot-specific reconstitution mass, target pH, and stability, consult the CoA/Spec Sheet. This product is designated For research use only.

Structure and Identity

Yeast Nitrogen Base Agar (YNB Agar) is a defined/dehydrated microbiological medium composed of a yeast nitrogen base premix solidified with agar. It is a formulated mixture rather than a single chemical entity.

• Item-specific identifiers from Product Data:

  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • InChIKey: Not applicable (complex mixture).
  • SMILES: Not applicable (complex mixture).
  • Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

• Structural features (general, literature):

  • Contains inorganic salts (e.g., ammonium, phosphate, sulfate, magnesium, etc.), vitamins/trace elements, and typically lacks amino acids and carbohydrates unless supplemented (literature—formulations vary by manufacturer).
  • Agar is a sulfated galactan polysaccharide providing a solid matrix; it contributes no defined small-molecule structure for representation.

• Description of 2D composition (general):

  • Multi-component blend: ionic species (salts), small organic micronutrients (vitamins), trace metals/chelates, and high–molecular-weight agarose/agalactan fibers.

• Nomenclature notes (general):

  • “Yeast Nitrogen Base (YNB)” denotes the basal salts/vitamins/trace elements mix. “YNB Agar” indicates YNB precombined with agar for plate preparation. A fermentable carbon source (e.g., glucose) is typically added separately depending on application.

Synthetic Utility

Not applicable as a chemical reagent. Yeast Nitrogen Base Agar is not used as a substrate, catalyst, or solvent in synthetic organic or inorganic reactions.

• Context for researchers (general):
  • If you are conducting chemoenzymatic or whole-cell biocatalysis on plates, this medium may provide a defined environment for screening yeast strains expressing enzymes of interest. However, the medium itself does not participate chemically; it merely supports cell growth.

For synthetic chemistry workflows, refer to dedicated reagents and solvents; this medium serves exclusively as a microbiological growth platform.

Target Specificity

This product is not a biological targeting reagent (e.g., antibody, probe) and has no antigen/epitope specificity.

• Item-specific data:
  • Antigen/epitope: Not applicable.
  • Species reactivity: Not applicable.
  • Clone/isotype: Not applicable.

Use this medium to culture yeast strains broadly; selectivity arises from supplements and nutrient omissions you define.