Experiments on the mutagenic effect of radiation on plant chromosomes
Understand the mutagenic effects of physical factors on genetic material through radiation on plants.
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Experiments on efficient Agrobacterium transformation methods in wheat mediated by in situ plant inoculation
This chapter details a reproducible method of Agrobacterium-mediated transformation of spring wheat by direct inoculation of Agrobacterium sap into immature see
Wheat Flower Organ Transformation Experiment
This chapter describes a method similar to the Arabidopsis inflorescence imbibition method for the transformation of wheat floral organs. Instead of relying on
Experiments on the pathways and rates of water transport in plants and the potential of vascular transport
Experiments to deepen understanding of the pathways and rates of water transport in plants and the potential for vascular transport.
Chromosome structure variation experiment
Understand that when chromosome structure is mutated, chromosome bridges or chromosome breaks can be observed in mitotic cells at later stages, and micronuclei
Micronucleus assay experiments on mutagenic substances
1. Understanding the methods and significance of micronucleus determination 2、Searching for new testing systems and new safer and more effective plant mutagen
Barley transformation experiment by gene gun method
Microparticle bombardment, also known as the gene gun method, has been widely used in the genetic transformation of cereal crops. This method brings DNA directl
Substantial equivalence (proteomics) experiments
Wheat, as one of the major global crops, is processed into a range of food products for consumer consumption. Therefore, it is of great importance to determine
Fluorescence in situ hybridization for the detection of transgene fragments integrated in plant genomes experiments
Over the past 30 years, fluorescent chromosome analysis techniques have facilitated the understanding of genome construction and deepened knowledge of the organ