3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Over the past 30 years, fluorescent chromosome analysis techniques have facilitated the understanding of genome construction and deepened knowledge of the organ
Wheat, as one of the major global crops, is processed into a range of food products for consumer consumption. Therefore, it is of great importance to determine
Agrobacterium-mediated transformation of barley has been widely utilized over the last decade and it offers advantages over gene gun-mediated transgenic techniq
With the development of transgenic technology systems, the transfer of heterologous genes into the plant genome has become possible, which provides a new avenue
The study of insertion patterns and loci of exogenous genes in transgenic plants greatly contributes to the understanding of the mechanisms of integration, expr
In Western Europe, for transgenic crops to be approved for commercial production, proof of their substantial equivalence to conventionally bred crops needs to b
Within this chapter, we present a protocol for extracting total protein from wood-forming tissues (secondary xylem in differentiation). This protocol has been u
Seeds may contain different components, like starch and polysaccharides, which can severely reduce the amount of protein extracted. The proteins in cereal plant
Another feasible method for protein extraction, besides the classical trichloroacetic acid (TCA ) /acetone precipitation method, is phenol extraction, which can
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