Protein extraction experiments on woody plants
Within this chapter, we present a protocol for extracting total protein from wood-forming tissues (secondary xylem in differentiation). This protocol has been u
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Root meristem cell nuclear protein extraction experiment
The isolation and extraction of nuclear proteins has facilitated the study of plant proteomics. This technique relies on subcellular isolation with which the pr
Xylem and phloem sap protein extraction experiments
It is well known that the xylem and primordial vasculature in higher plants are responsible for the long-distance transport of small nutrient molecules. However
Plant whole protein extraction by trichloroacetic acid-acetone method
In this chapter we describe an efficient method suitable for whole protein extraction from various plant tissues. The method utilizes trichloroacetic acid and a
Experimental proteomic analysis of Arabidopsis thaliana chloroplasts
This chapter describes a simple and practical method for large-scale isolation and purification of chloroplasts. The advantages of this method are high yield, l
Experiments on the quantitative analysis of two-dimensional gels
Two-dimensional electrophoresis, as an important experimental tool for comparative proteomics studies, has been applied to conveniently reflect the differences
Extraction and solubilization experiments of plant plasma membrane proteins
In all living cells the plasma membrane (PM ) exists as an interface between the cytoplasm and the environment and is one of the most complex and differentiated
Alfalfa stem cell wall protein extraction experiment
The plant cell wall is a highly dynamic and chemically active component of the plant cell. The main components of the cell wall are polysaccharides, as well as
Bidirectional gel fluorescence staining and imaging experiments
Staining of biphasic gel electrophoresis is a crucial step in comparative proteomics studies regarding the number of proteins to be measured, the accurate quant