3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Because of its outstanding ability to separate total proteins, bidirectional gel electrophoresis can be applied to the separation of all types of proteins. Howe
The integrity of the proteome of subcellular components such as the nucleus is largely determined by whether or not other cellular contaminants are removed from
Two-dimensional electrophoresis, as an important experimental tool for comparative proteomics studies, has been applied to conveniently reflect the differences
PROTICdb is a web-based database designed primarily to store and analyze plant proteomic data obtained by 2D polyacrylamide electrophoresis and mass spectrometr
Prior to the identification of membrane proteins by matrix-assisted laser-resolved time-of-flight mass spectrometry (MALDI-TOF-MS), these proteins need to be se
MALDI-TOF peptide mass fingerprinting (PMF) is a faster and more convenient method for identifying proteins. The genomes studied have been sequenced and annotat
Like other eukaryotic cells, glycosylation is one of the most studied post-translational events in plant cells. Depending on the type of connection between the
In this chapter, we present a detailed experimental method for the large-scale identification of proteins in rice leaf and root tissues using 2D liquid chromato
Immunoblotting using polyvinylidene difluoride (PVDF ) membranes is the most commonly used method for Edman sequencing. Protein samples are transferred from a 2
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