Separation of T cells and B cells by magnetic bead method
This unit describes the separation of T cells, B cells, and T cell subpopulations by magnetic separation techniques. Author: J.E. Collier et al, Translator: Xui
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Detection of lymphocyte migration and proliferation using the intracellular fluorescent dye CFSE
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Induction and detection of cytotoxic T lymphocytes
This unit describes several experimental protocols for inducing cytotoxic T lymphocytes and assaying their activity Remember that, in principle, any kind of cel
B Proliferation assays for cell function
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Human α2a, α2b Interferon Assay
An indispensable part of the production process of recombinant human α2a/α2b interferon is the quality control of the product, and the bioactivity o
BrdU assays for d-cell and B-cell proliferation
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Establishment of T-cell clones
This unit provides a method for establishing specific T cell clones. Note that the key to the success of this method is the selection and preparation of conditi
Hydrolysis of immunoglobulin G (IgG) fragments
When a new antibody needs to be fragmented, it is often necessary to perform a touch-and-go test. When choosing the reaction time, it is preferable to keep part
Cloning, Expression and Modification of Antibody Variable Regions (V Regions)
PCR-based cloning schemes utilize conserved sequences in the framework and pilot regions of the variable region, and although primers can be synthesized with co