Detection of IL-18
Newly synthesized IL-18 is a precursor molecule to the signal peptide, which is enzymatically cleaved in order to activate it. In this ELISA method, in order to
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Detection of cytokine secretion and isolation of cytokine-secreting cells by flow cytometry
This protocol is for the detection of cytokine production by activated antigen-specific T cells in vitro. If other stimulants or stimulated cells are used in vi
IL-12 detection assay
This is a method for the specific detection of IL-12 heterodimers. Since IL-12 p4 0 monomer cannot be detected, this method is able to detect IL-12 p7 5 heterod
IL-10 detection assay
This protocol uses an antibody specific for mouse IL- 1 0 in the E LISA method to detect mouse IL- 1 0 , and does not detect human or viral IL-10. IL-10 from ot
Gamma interferon assay
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Isolation of mouse macrophages
Introduction of a method for the isolation of mouse macrophages by J.E. Colligan et al, translated by Xuitao Cao et al. This experiment is from the "Compendium
Detection of anti-tumor activity of macrophages
Mouse macrophage cell lines with tumor cell killing activity, such as A N A -1 and G G 2E E . Mouse primary macrophages can be freshly isolated. Author: J.E. Co
Phenotypic assay of mouse macrophages
Due to the lack of characteristic surface markers of macrophages or macrophages at different stages of differentiation, macrophages are currently identified bas
Detection of α-, β- and γ-interferon-induced antiviral activity
The cells used in the assay are mouse L 929 fibroblasts, which have a high sensitivity to interferon and V S V . The cells should be derived from laboratories t