Detection of tyrosine protein kinase by immunocomplex methods
The low abundance of tyrosine protein kinase (T P K ) expression and its rapid activation and inactivation make it difficult to purify using conventional bioche
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3228 articles
Analysis of inositol phospholipid turnover during lymphocyte activation
Receptor-mediated activation of phospholipase C (P L C) induces hydrolysis of membrane inositol phospholipids to produce diacylglycerol (D A G) and water-solubl
Blotting assay for anti-phosphorylated tyrosine
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Analysis of mitogen-activated protein kinase (MAPK) activity in T cells
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Preparation of mouse T-cell hybrid tumors
T-cell hybridomas can be obtained by fusing activated T cells with tumor cells. Heterogeneous hybridomas can be cloned by limited dilution to obtain hybridomas
Detection of cytokine secretion and isolation of cytokine-secreting cells by flow cytometry
This protocol is for the detection of cytokine production by activated antigen-specific T cells in vitro. If other stimulants or stimulated cells are used in vi
Detection of IL-18
Newly synthesized IL-18 is a precursor molecule to the signal peptide, which is enzymatically cleaved in order to activate it. In this ELISA method, in order to
ELISPOT method for detection of cytokine-secreting cells
Enzyme-linked immunospot test (ELISPOT) is based on the principle that cells that secrete certain protein molecules (e.g. cytokines) are able to secrete a relat
Gamma interferon assay
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".