B Identification of functional subpopulations of cells
Peripheral mature B cells can be divided into several subpopulations. Traditionally, B cells have been divided into two subpopulations, CD5+ B1 cells and CD5 B2
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B Cell activation
B cells are activated and proliferate upon activation by specific antigens, anti-Ig antibodies, and/or mitogens.
Natural killer cell clearance in mice
NK cells are usually injected intravenously; in vivo clearance can be achieved by intraperitoneal injection of antibodies against NK cells, such as PK136 monocl
Cultured dendritic cells from mouse bone marrow precursor cells
Dendritic cells (DCs) are very few in number in vivo (less than 1% of splenic adherent cells), and the time-consuming isolation of DCs from vivo and their low c
Macrophage chemotaxis assay
Macrophage migration is the primary condition for its immune activity at the site of infection and injury, and macrophage chemotaxis reflects its migration abil
Monoclonal antibody identification test
The identification of monoclonal antibodies is similar to that of polyclonal antibodies, and the methods used are common; however, the identification of some of
Isolation and purification of human metaphase immune cells
The metaphase immune cell population is the biological basis of maternal-fetal immune tolerance, and its composition is extremely special, mainly composed of sp
Detection of antinuclear antibodies in serum by immunofluorescence technique assay
Antinuclear antibody (ANA) is an autoantibody directed against the nuclear components of cells, with no organ or germline specificity. A positive ANA is indicat
Chemiluminescence immunoassay experiment
Chemiluminescence (CL) refers to the phenomenon of light radiation that accompanies the process of a chemical reaction. Most chemiluminescence is the luminescen