Preparation of monoclonal antibodies
Hybridoma cells are formed by fusing an immune animal B lymphocyte that produces a single antibody with myeloma cells by cell fusion techniques (e.g., PEG-induc
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Multi-parameter data acquisition and analysis experiments on leukocytes
Several software packages are now available for flow cytometry data analysis. Although each one has a different approach in processing the data, the basic strat
Flow cytometric analysis of fluorescence resonance energy transfer
The study of various cellular life processes has been made easier by the detection of intermolecular interactions in vivo and in vitro through a variety of meth
Analysis of DNA content in solid tumors
Flow cytometry DNA content assays provide a rapid and credible method of analyzing DNA ploidy and cell proliferation scores in tumor specimens. Both preserved a
Characterization of hematopoietic stem cells
Hoechst 33342 and Rhodamine123 Staining for Hematopoietic Stem Cell Characterization Given that the in vitro in vivo dyes Rhodamine123 (Rh123) and Hoechst 33342
Detection and enrichment of hematopoietic stem cells by marginal group cell phenotyping
Detection and enrichment of hematopoietic stem cells by marginal group cell phenotyping is based on the isolation of hematopoietic stem cells from mouse bone ma
Combined application of flow cytometry and gene chip methods to detect transcriptional profiles
The two technologies, flow cytometry and gene expression profiling, share many common features: they involve complex, highly sophisticated equipment; they can p
Mammalian protein-protein interaction traps based on fluorescence-activated cell sorting technology
The Mammalian Protein-Protein Interaction Trap System (MAPPIT) is a two-hybrid system designed based on the understanding of the signaling mechanism of type I c
Frequency of antigen-specific T-cell precursor proliferation detected using cellular tracer dyes
T cells recognize specific antigenic molecules (epitopes) through T cell antigen recognition receptors, and although the body's T cell pool can respond to a var