Induction and isolation of human blood mononuclear macrophages
Macrophages (MΦ) are mature cells formed by the migration of blood monocytes into tissues, and are widely distributed in the spleen, liver, abdominal cavity, n
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Isolation and purification of human metaphase stromal cells
Endometrial metamorphosis is a critical step for embryo implantation and successful pregnancy. The cellular composition of the metaphase tissue is quite complex
Measurement of immune cell phagocytosis
The body has phagocytosis (phagocytosis) function of the cells called phagocytes, including monocyte macrophages and neutrophils two categories. Phagocytes phag
CFSE assay for human peripheral blood T cell expansion in vitro
CFSE detection of human peripheral blood T cell expansion in vitro refers to the use of CFSE to fluorescently label live cells, which can be used for the detect
B Phenotypic detection of cells
There are many membrane molecules on the surface of B cells, including cell surface differentiation antigen and model immunoglobulin (smlg). At different stages
Antibody potency assay
Antibody potency assay is used to test the potency of immune serum. Currently there are three main methods used to determine antibody potency: tube agglutinatio
In vivo activation of macrophages
Macrophages are important natural immune cells in vivo, and in vitro detection of their function does not fully reflect their in vivo behavior, while detection
Isolation of peripheral blood single nucleated cells
Immune cell isolation techniques are developing in the direction of more and more "fine", immune cell subpopulation and even micro-immune cell isolation and ide
Evaluation of lymphocyte-mediated cytotoxicity using flow cytometry
Cytotoxicity of lymphocytes, is the killing of target cells by the release of particles containing perforin and granzyme and/or through the interaction of Fas-F