Leukocyte classification and counting test
The blood is made into a thin film smear with uniform cell distribution, stained with complex dyes, classified and counted according to the morphological charac
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
MTT-based cytotoxicity assay
It is mainly used to detect the killing activity of effector cells on target cells.
Chloramphenicol acetyltransferase (CAT) assay experiment
By determining the expression of reporter genes, such as β-gal or CAT, in transfected cells, it can be determined whether or not the guide DNA has been integra
Primary cell culture-mouse embryo isolation experiment
Primary cell culture can be applied to (1) molecular biology; (2) cell biology; (3) genetics; (4) immunology; (5) oncology; (6) virology and other fields.
Resuscitation of frozen cells experiment
Rapidly resuscitate cells, slowly dilute and re-inoculate at high cell density Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition
Epidermal keratin-forming cell model construction experiment
Cultured epidermal keratinocytes are used as (1) models of differentiation, ideal tissue constructs for organotypic cultures, and studies of cell-cell interacti
Primary renal epithelial cell culture experiment
Renal epithelial cell cultures can be used: (1) to study the Na+-dependent inorganic phosphorus transport system inhibited, for example, by parathyroid hormone;
Experimental induction of human mesenchymal stem cell immortalization with telomerase
Telomerase plays an extremely important role in the stability of chromosomes and in determining the cellular life cycle, and is mainly used in (1) the developme
spheroid culture (biology)
Digest monolayers of cells or disperse the original tissue with trypsin and inoculate the cells onto a substrate coated with agar. The aggregates are transferre