Alginate coating
Cells in 75 cm2 culture flasks were digested with trypsin, counted and mixed with alginate solution. Alginate cell suspension was added dropwise to calcium chlo
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Culture of colorectal tumor cells
The adenoma is usually digested with enzymes, and the cancerous tissue is minced with a scalpel blade. If well-differentiated colorectal cancer specimens are no
density gradient cell separation method
This experiment is mainly used to isolate cells.
Osteoblast primary culture experiment
This experiment was used for primary culture of osteoblasts.
Primary culture of adipocytes
This experiment is mainly used for primary culture of adipocytes.
Long-term culture of bone marrow hematopoietic cells
Bone marrow is aspirated into the culture medium. The bone marrow is maintained as an attached multilayer of cells for at least 12 weeks and up to 30 weeks. Ste
Report gene: in situ staining assay for beta-galactosidase
In situ staining for β-galactosidase can be applied (1) to determine whether the guide DNA has been integrated into the host cell and (2) to trace cell-cell in
Hematopoietic cell colony formation assay
Bone marrow cells were suspended with agar or methylcellulose, and then the cells were inoculated in Petri dishes with appropriate growth factors.
Sterilization of filtration units
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)