Separation of mouse embryos
Isolation of mouse embryos can be used for: (1) Culturing mouse embryos in vitro and figuring out appropriate culture conditions. (2) Using embryos for developm
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3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Petri dish
Cells were inoculated into filter membrane Petri dishes and cultured in excess culture solution in multiwell culture plates.
rotary flask culture (chemistry)
Add the cell suspension to a round culture flask and then place the flask on a rotating rack and turn it slowly.
nerve aggregates
Brains were removed from fetal rats at 17 d or 18 d of gestation (contact the local animal ethics committee) and the brains were made into single cell suspensio
Stable transfection by electroporation
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
microcarrier
Cells and microbeads were inoculated at high concentrations and then diluted, stirred and sampled as required.
Inoculation of suspension cells in cell culture flasks
Inoculation of suspension cells in cell culture flasks can be used for (1) mass culture of cells and (2) providing cells with a suitable environment for growth.
serological testing
Different lots of serum have different abilities to support cell growth, especially clonal cell growth. Testing different batches of serum and then purchasing s
Isolation and culture of mouse keratinocytes
This protocol is a modification of the force method of Hennings et al. (1980). Gestational BALB/c mice are available from Harland Sprague Dawley, and the protoc