Vibrant Coloring
Various cellular manipulations, including passaging, freezing, and isolation of primary tissues, can lead to cell death. To determine the number of surviving ce
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Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
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Trypsin treatment of monolayer cells
The following protocol describes the use of trypsin to treat cells for passaging or collection. Cell lines are usually maintained by passaging once a week and c
Resuscitation of frozen cells
The resuscitation of frozen cells can be used for: (1) In the experimental manipulation of cells, frozen cells are resuscitated and then cultured for passaging.
Isolation of fibroblasts
The generalized protocol described below applies to mouse, rat, hamster, and chicken embryos. It is best to select embryos that are approximately half the time
Inoculation of monolayer cells in cell vials
Cells growing in monolayers proliferate, fuse into sheets, and grow all over the surface of the cell vial. Some cells can be maintained in the plateau phase for
Isolation and culture of bovine keratinized epithelial cells
This protocol is based on the method of Ehaio et al. (1987) and can be modified for the isolation and culture of other epithelial cells if tissue is available.
Reduces contamination during epithelial cell culture
Epithelial cells are susceptible to fibroblast contamination, the extent of which can be determined by double immunofluorescence staining using antibodies speci
blood count
Blood count analysis can be used to (1) get basic information about the patient's blood and (2) find out if the patient has an infection, anemia, or coagulation
Fluorescence microscopy for mycoplasma detection
There are two different strategies for detecting mycoplasma: the first method, which will be described in the following scheme, utilizes the fluorescent dye Hoe