Isolation of suspension cell clones
Aspirate colonies with a spiking gun or Pasteur pipette and transfer to culture wells in culture flasks or multiwell plates.
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Mammary epithelial cell culture experiment
Breast milk and mastopexy excised tissue are suitable sources of material for normal mammary ductal epithelium, and purer epithelial cells can be obtained from
Culture of cerebellar granule cells
Cerebellums from 4-8 neonatal rats were cut into small cubes, placed in HBSS, and digested with trypsin for 15 min at 37°C. Cells were inoculated into petri
organ culture experiment
Since the desired organ culture technique involves placing the tissue in a position ideal for gas and nutrient exchange, most of these techniques involve placin
Melanocyte culture
After trypsin digestion, epidermal slices isolated from skin slices were dispersed with EDTA and then incubated with serum-free culture medium supplemented with
Olfactory bulb sheath-forming cells
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Density-limiting experiments on cell proliferation
Both cultured and in vitro transformed cells from tumors exhibit abnormalities in growth control, such as higher saturation densities, formation of colonies in
Separation of cell colonies by radiation
Place the culture bottles upside down under an X-ray machine or 60Co source and shade the desired colonies with a lead block.
Cloning culture in Mexisol
Cells were suspended in medium containing Mexisol, and such cells were seeded in Petri dishes lined with an agar (or agarose) gel base.