Clonal culture in agar
The agar is liquid at high temperatures, but becomes a gel at 37°C. Cells are suspended in warm agar gel medium and cultured after the agar has formed a gel, f
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Separation of cell clones by cloning rings
Colonies were digested with trypsin in porcelain cloning rings, glass rings, PTFE rings, or stainless steel rings and transferred to one well of a 24-well or 12
Experiments on the isolation and culture of colonic crypts
Although there have been many reports of continuous cell lines for human colon cancer, there are fewer reports of normal intestinal epithelial cultures.Owens et
Giemsa stain
Cultures were fixed with methanol and stained directly with undiluted Giemsa's solution, then the stain was diluted 1:10, the cultures were washed, and observed
Preparation and sterilization of glassware
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Microscope digital photography
Open the microscope and calibrate the optical path. Focus on the relevant area of the specimen. Check the connection between the camera and the computer, direct
DNA assay with Hoechst 33258
Homogenize cells or tissues in buffer, followed by sonication. An amount of the cell suspension is mixed with Hoechst 33258 and the fluorescence is determined.
Crystalline violet staining
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Methotrexate resistance and DHFR amplification
The cells are sequentially placed in a series of concentrations of MTX for several weeks, with periodic changes to fresh medium containing the same drug concent