Preparation and Sterilization of D-PBSA
The dry powder was dissolved by constant stirring, adjusted to final volume, tested for pH and conductivity, and autoclaved in predetermined portions.
Knowledge Base
Laboratory Protocols & SOPs Library
3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
3228 articles
Magnetic Activated Cell Sorting (MACS)
A buffy coat or another mixed cell suspension is mixed with antibody-conjugated microbeads, diluted and placed in a magnetic separation column. Cells bound to t
Use of the inverted microscope
Place the culture on the microscope's stage, turn on the power, select the appropriate lens and focus on the specimen. Adjust the condenser and phase contrast r
Chromosome Preparation
Cells blocked in mid-division were fixed, swollen in hypotonic solution, dropped on slides, stained, and observed [ Rothfels and Siminovitch, 1958; Rooney and C
Cell counting experiments with blood cell counting plates
Digest the monolayer culture with trypsin, or take a sample from the suspension culture; prepare a coverslip and add the cells to the small chamber of the hemoc
Clean Incubator
Move the culture to another incubator, turn off the power to the empty incubator, then scrub it with detergent and ethanol and turn on the heat switch in order
Filtration with syringe-type filtration for bacteria transport
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Experiments for plotting growth curves of cells cultured in monolayers in culture flasks
Following passaging culture, cells assume growth forms characterized by delayed, exponential or logarithmic, and stationary (plateau) phases. The logarithmic an
Experiments for drawing growth curves of cells cultured in monolayers in multiwell culture plates
Three sets of cells were cultured in multiwell plates at three different cell concentrations, and cells in one plate were counted each day before reaching plate