Cell viability assay by dye rejection
Viability assays are used to detect potentially damaging processes such as primary isolation, cell separation, or the percentage of living cells after freeze-th
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Cell viability assay by dye uptake assay
Living cells can take up diacetylfluorescein and hydrolyze it to fluorescein, which cannot pass through the cell membrane of living cells [Rotman and Papermaste
Dialysis of serum
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Labeling index experiments with 3H-thymine deoxyriboside
Cells are cultured to the appropriate density, labeled with 1H -thymidine deoxyriboside for 30 min, washed and fixed, and unadulterated precursors are removed i
primary outgrowth
The tissues were minced and washed, and the tissue blocks were inoculated on the surface of culture flasks or Picot dishes with a small amount of medium contain
Oral keratinocytes
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Elimination of microbial contamination
Cultures were washed several times with high concentrations of antibiotics by rinsing monolayer cultures or by resuspending non-adherent cells by centrifugation
Detection of HLA-B27 Expression
This experiment is based on "Color Atlas of Practical Flow Cytometry", edited by Shukui Wang and Zhenying Zhou.
Conversion of the EB virus into lymphocytes
This experiment is based on the "Guide to Cellular Experiments", translated by Huang Peitang et al.