Guanidine HCl - 10mM in DMSO , CAS No.50-01-1

CAS: 50-01-1 Cat. No.: G408589 Formula: CH₅N₃ · HCl Peso molecolare: 95.53 Beilstein Registry Number: 3591990 Numero EC: 200-002-3

GRADE & PURITY 10mM in DMSO

Synonyms

Aminoformamidine HCl | Guanidine, hydrochloride (1:1)

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Panoramica

Information

Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.
In vitro

Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by Guanidine HCl provides several important parameters regarding the mechanism of conformational stability of proteins. Guanidine HCl at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. Guanidine HCl (> 1 M) causes co-operative unfolding of the molten globule state. Guanidine HCl is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △G^H₂O_app. values of Guanidine HCl are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. Guanidine HCl at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI⁺] element from yeast cells. 5 mM Guanidine HCl in growth media cures [PSI⁺] and other prions of yeast. 5 mM Guanidine HCl significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine HCl also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase.

In vivo

Cell Data

cell lines：HT29-S-B6 colon cell

Concentrations：

Incubation Time：

Powder Purity:≥100%

Specifications

Sinonimi

Aminoformamidine HCl | Guanidine, hydrochloride (1:1)

Specifiche e purezza

10mM in DMSO

Meccanismi biochimici e fisiologici

Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.

Condizioni di conservazione di stoccaggio

Store at -80°C

Spedito in

Dry ice packs + Cold packs

Questo prodotto richiede spedizione a catena fredda. I servizi di terra e altri servizi economici non sono disponibili.

Nomi e identificatori

• Isomeri SMILES: C(=N)(N)N.Cl
• WGK Germania: 1
• RTECS: MF4300000
• Peso molecolare: 95.53
• Beilstein: 3591990
• Reaxy-Rn: 3591990
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3591990&ln=

Proprietà chimiche e fisiche

• Punto di fusione (°C): 178-185°C

Domande frequenti

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 50-01-1, the molecular formula is CH5N3 · HCl, and the molecular weight is 95.53 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.