ADIPOQ Mouse mAb - Primary antibody, for WB,IHC various applications, from Human, generated in Mouse, specific to ADIPOQ, Mouse IgG2b

Cat. No.: Ab087546

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, See COA

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Pig, Rat

Isotype

Mouse IgG2b

Clone Number

H2

Conjugation

Unconjugated

Visão geral

Mouse monoclonal (H2) to ADIPOQ could be used for WB, IHC and so on.
Application
WB: 0.29-2µg/mL;
IHC: 5-20µg/mL;
Protein Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Post-translational
Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation.

Specifications

Product Name

ADIPOQ Mouse mAb - Primary antibody, for WB,IHC various applications, from Human, generated in Mouse, specific to ADIPOQ, Mouse IgG2b

Sinónimos

Adiponectin antibody | 30 kDa adipocyte complement related protein antibody | 30 kDa adipocyte complement-related protein antibody | ACDC antibody | Acrp 30 antibody | ACRP30 antibody | ADIPO_HUMAN antibody | Adipocyte antibody | Adipocyte C1q and collage

Especificações e pureza

Carrier Free, ExactAb™, Validated, High Performance, See COA

Espécies hospedeiras

Mouse

Espécie hospedeira

Mouse

Especificidade

ADIPOQ

Aplicação

WB,IHC

Imunogénio

Recombinant ADIPOQ (Ala108~Asn244) expressed in E.coli

Controlo positivo

WB: K562, HeLa and C6 cell lysates. IHC: Human Cardiac Muscle Tissue.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, High Performance, Validated

Tipo de anticorpo

Primary antibody

Clonalidade

Monoclonal antibodies

Isótipo

Mouse IgG2b

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A + Protein G affinity chromatography.

Fonte

Hybridoma supernatant

Concentração

See COA

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4 ℃ for frequent use. Aliquot and store at -20 ℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: ADIPOQ Mouse mAb (Ab087546)

ADIPOQ Mouse mAb (Ab087546) - Western Blot
All lanes: ADIPOQ Mouse mAb (Ab087546) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 26 kDa
Observed band size: 25, 27 kDa
Exposure time: 80.6 s

IHC: ADIPOQ Mouse mAb (Ab087546)

ADIPOQ Mouse mAb (Ab087546) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL ADIPOQ Mouse mAb (Ab087546)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Aplicação

• Aplicação: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.29-2µg/mL;

Alvos associados (humanos)

ADIPOQ Tbio Adiponectin (0 Activities)

Discover Target: ADIPOQ

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (ADIPOQ Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.