Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, for ELISA,LF,GICA various applications, from Mouse, generated in Goat

Cat. No.: Ab115835

GRADE & PURITY Carrier Free, Validated, ExactAb™, See COA

Application

ELISA, GICA, LF

Host species

Goat

Reactive species

Mouse

Conjugation

Unconjugated

Overview

Goat Anti-Mouse IgG H&L Antibody, Secondary antibody, could be uesd for ELISA, LF, GICA and so on.

Specifications

Product Name

Goat Anti-Mouse IgG H&L Antibody - Secondary antibody, for ELISA,LF,GICA various applications, from Mouse, generated in Goat

Synonyms

Goat Anti-Mouse IgG H&L Antibody | Goat Anti Mouse IgG H&L Antibody | Anti-Mouse IgG H&L Antibody | Anti Mouse IgG H&L Antibody | Mouse IgG H&L Antibody | Goat Anti-Mouse IgG Antibody | Goat Anti Mouse IgG Antibody | Anti-Mouse IgG Antibody | Anti Mouse I

Specifications & Purity

Carrier Free, ExactAb™, Validated, See COA

Host Species

Goat

Host species

Goat

Application

ELISA, LF, GICA

Immunogen

Mouse IgG

Positive Control

Mouse IgG

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Antibody Type

secondary antibody

Clonality

polyclonal antibodies

SDS-PAGE

150kDa

Purification Method

Antigen affinity purified

Source

Serum

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C long term (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

IF: Goat Anti-Mouse IgG H&L Antibody (Ab115835)

Goat Anti-Mouse IgG H&L Antibody (Ab115835) - IF
IF analysis of beta Tubulin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Tubulin Mouse mAb (Ab155823) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L Antibody (Ab115835) conjugated with biotin at a dilution of 1/1000 for 1 hour at room temperature in the dark. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at a dilution of 1/200 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: Goat Anti-Mouse IgG H&L Antibody (Ab115835)

Goat Anti-Mouse IgG H&L Antibody (Ab115835) - Flow Cytometry
Flow analysis of beta Actin (red line) in Jurkat cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/5000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L Antibody (Ab115835) conjugated with FITC (Fluorescein isothiocyanate) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Application

• Application: Dilution info
• ELISA:

  Use at an assay dependent concentration.;

• LF:

  Use at an assay dependent concentration ;

• GICA:

  Use at an assay dependent concentration.

References

1. Xu Tao, Yang Changwei, Lu Yang, Wang Heng, Chen Cheng, Zhou Yuchen, Chen Xiaoqing.  (2024)  Photocrosslinkable human amniotic membrane hydrogel for recovery from spinal cord injury.  Bio-Design and Manufacturing,      [PMID:] [10.1007/s42242-024-00318-x]