AIMP3/p18 Mouse mAb - Primary antibody, specific to EEF1E1, Mouse IgG2b

Cat. No.: Ab087803

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Mouse

Reactive species

Human, Mouse

Isotype

Mouse IgG2b

Clone Number

E94S639

Conjugation

Unconjugated

Visão geral

Mouse anti Human AIMP3/p18 Antibody, Monoclonal (E94S639), could be used for WB, IF/ICC and so on.

Specifications

Product Name

AIMP3/p18 Mouse mAb - Primary antibody, specific to EEF1E1, Mouse IgG2b

Sinónimos

AIMP 3 | AIMP3 | Aminoacyl tRNA synthetase complex interacting multifunctional protein 3 | Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 | ARS interacting multifunctional protein 3 | Cyclin dependent kinase 6 inhibitor | Elongati

Especificações e pureza

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Mecanismos bioquímicos e fisiológicos

Positive modulator of ATM response to DNA damage.

Espécie hospedeira

Mouse

Especificidade

EEF1E1

Imunogénio

Recombinant fusion protein of human EEF1E1.

Controlo positivo

WB: Mouse testis, Rat brain, Hep G2, A549, A431, Jurkat and K562 lysates. IF/ICC: HeLa cells.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, Validated

Isótipo

Mouse IgG2b

SDS-PAGE

150 kDa

Método de purificação

Protein G purified

Concentração

0.5 mg/mL

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: AIMP3/p18 Mouse mAb (Ab087803)

AIMP3/p18 Mouse mAb (Ab087803) - Western Blot
All lanes: AIMP3/p18 Mouse mAb (Ab087803) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 19 kDa
Exposure time: 12.0 s

Western Blot: AIMP3/p18 Mouse mAb (Ab087803)

AIMP3/p18 Mouse mAb (Ab087803) - Western Blot
All lanes: AIMP3/p18 Mouse mAb (Ab087803) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa
Observed band size: 19 kDa
Exposure time: 25.5 s

IF/ICC: AIMP3/p18 Mouse mAb (Ab087803)

AIMP3/p18 Mouse mAb (Ab087803) - IF/ICC
Immunofluorescent analysis of EEF1E1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with AIMP3/p18 Mouse mAb (Ab087803) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

Aplicação

• Aplicação: Dilution info
• ICC/IF:

  1/10-1/50 ;

• WB:

  1/500-1/2000 ;

Alvos associados (humanos)

EEF1E1 Tbio Eukaryotic translation elongation factor 1 epsilon-1 (0 Activities)

Discover Target: EEF1E1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (AIMP3/p18 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.