GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
Functions as an adapter recruiting ubiquitin-protein ligases to their specific substrates.Through an ubiquitination-dependent mechanism plays for instance a role in the incorporation of SLC11A2 into extracellular vesicles. More generally, plays a role in
Espécie hospedeira
Rabbit
Especificidade
ARRDC1
Imunogénio
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ARRDC1.
Controlo positivo
WB:HeLa, NIH/3T3 and C6 cell lysates. IF/ICC: MDA-MB-231 cells.
Conjugação
Unconjugated
Grau
Carrier Free, ExactAb™, Validated
Isótipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificação
Immunogen affinity purified
Concentração
1.0 mg/mL
Armazenamento e envio
Forma
Liquid
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imagens
ARRDC1 Antibody (Ab089692) - Western Blot All lanes: ARRDC1 Antibody (Ab089692) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 45 kDa Observed band size: 48 kDa Exposure time: 86.8 s
ARRDC1 Antibody (Ab089692) - IF/ICC Immunofluorescent analysis of ARRDC1 staining in MDA-MB-231 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ARRDC1 Antibody (Ab089692) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Aplicação
Aplicação
Dilution info
WB
1/500-1/2000
IF/ICC
1/100-1/500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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