Goat Anti-Rabbit IgG H&L (Cy5) - Secondary antibody

Cat. No.: Ab170147

GRADE & PURITY Validated, ExactAb™, Azide Free, Ex:647nm, Em:665nm, Pre-adsorbed, 1.0 mg/mL

Application

IF/ICC

Host species

Goat

Reactive species

Rabbit

Conjugation

Cy5

Overview

Application
IF: 1:1000

Specifications

Product Name

Goat Anti-Rabbit IgG H&L (Cy5) - Secondary antibody

Synonyms

Goat Anti-Rabbit IgG H&L Antibody (Cy5) | Anti-Rabbit IgG H&L Antibody (Cy5) | Rabbit IgG H&L Antibody (Cy5) | Cy5-conjugated Goat Anti-Rabbit IgG (H+L) | Goat Anti Rabbit IgG Antibody (Cy5) | Anti-Rabbit IgG Antibody (Cy5) | Rabbit IgG Antibody (Cy5) | G

Specifications & Purity

ExactAb™, Azide Free, Validated, Ex:647nm, Em:665nm, Pre-adsorbed, 1.0 mg/mL

Host species

Goat

Immunogen

Rabbit IgG

Conjugation

Cy5

Excitation (nm)

Red Laser(633nm)

Emission(nm)

665nm

Ex/Em(nm)

Ex:647nm;Em:665nm

Grade

Azide Free, Em:665nm, Ex:647nm, ExactAb™, Pre-adsorbed, Validated

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20°C for one year. Avoid repeated freeze/thaw cycles. Protect from light.

Images

IF: Goat Anti-Rabbit IgG H&L (Cy5) (Ab170147)

Goat Anti-Rabbit IgG H&L (Cy5) (Ab170147) - IF
IF analysis of beta Actin (red) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Antibody (Ab091004) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (Cy5) (Ab170147) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (blue). PBS instead of the primary antibody was used as the secondary antibody only control. Images were taken on the confocal laser scanning microscope.

Application

• Application: Dilution info
• IF/ICC:

  1:1000;