GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Visão geral
Rabbit anti Human ARRDC3 Antibody, Polyclonal, could be used for WB, IHC and so on.
Specifications
Product Name
ARRDC3 Antibody - Primary antibody, specific to ARRDC3, Rabbit IgG
Sinónimos
ARRDC 3 antibody | Arrestin domain containing 3 antibody | Arrestin domain containing protein 3antibody | KIAA1376antibody | TBP-2-like inducible membrane protein antibody | Thioredoxin binding protein 2 like inducible membraneantibody | TLIMPantibody | A
Especificações e pureza
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
Mecanismos bioquímicos e fisiológicos
Adapter protein that plays a role in regulating cell-surface expression of adrenergic receptors and probably also other G protein-coupled receptors (PubMed:20559325, PubMed:21982743, PubMed:23208550). Plays a role in NEDD4-mediated ubiquitination and endo
Espécie hospedeira
Rabbit
Especificidade
ARRDC3
Imunogénio
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ARRDC3.
Controlo positivo
WB: MCF7 cell lysate. IHC: Human brain tissue. IF/ICC: HeLa cells.
Conjugação
Unconjugated
Grau
Carrier Free, ExactAb™, Validated
Isótipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificação
Immunogen affinity purified
Concentração
1.0 mg/mL
Armazenamento e envio
Forma
Liquid
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imagens
ARRDC3 Antibody (Ab089693) - Western Blot All lanes: ARRDC3 Antibody (Ab089693) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 46 kDa Observed band size: 50 kDa Exposure time: 2.9 s
ARRDC3 Antibody (Ab089693) - IHC Immunohistochemical analysis of ARRDC3 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ARRDC3 Antibody (Ab089693) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ARRDC3 Antibody (Ab089693) - IF/ICC IF analysis of ARRDC3 Antibody (Ab089693) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with ARRDC3 Antibody (Ab089693) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).
Aplicação
Aplicação
Dilution info
IF/ICC
1/200
WB
1/500-1/1000
IHC
1/50-1/100
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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