MAb de ratinho ASPM - Primary antibody, specific to ASPM

Cat. No.: Ab089860

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2b

Clone Number

K52002_11G4

Conjugation

Unconjugated

Visão geral

O anticorpo anti ASPM humano de coelho, monoclonal (K52002_11G4), pode ser utilizado para WB, IHC e assim por diante

Specifications

Product Name

MAb de ratinho ASPM - Primary antibody, specific to ASPM

Sinónimos

ASPM | ASP | MCPH5 | Calmbp1 | Asp (fuso anormal) homolog, anticorpo associado à microcefalia | Asp (fuso anormal) like, anticorpo associado à microcefalia | anticorpo ASP | anticorpo Calmbp 1 | anticorpo Calmbp1 | anticorpo Calmodulin binding protein 1 |

Especificações e pureza

ExactAb™, validado, sem suporte, 1,0 mg/mL

Espécie hospedeira

Mouse

Especificidade

ASPM

Imunogénio

Recombinant protein of human ASPM (AA 411-720)

Controlo positivo

WB: HEK293 lysate. IHC: Human adrenal gland tissue.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, Validated

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

1.0 mg/mL

Armazenamento e envio

Forma

Líquido

Condições de armazenamento de armazenamento

Conservar a -80°C. Evitar a congelação e descongelação repetidas

Enviado em

Dry ice packs + Cold packs

Estabilidade e armazenamento

Armazenar a 2-8 ℃ (3 meses). Armazenar a -20 ℃ (12 meses). Armazenar a -80 ℃ (36 meses). Após a receção, recomenda-se a aliquotagem. Evitar o ciclo de congelamento/descongelamento.

Imagens

Western Blot: ASPM Mouse mAb (Ab089860)

ASPM Mouse mAb (Ab089860) - Western Blot
15 μg of HEK293 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. ASPM Mouse mAb (Ab089860) at 1 μg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse lgG was used as the secondary antibody. ASPM band was visualized using ECL Substrate.
Result: ASPM Mouse mAb (Ab089860) can detect ASPM by Western blotting.
Predicted band size: 410 kDa
Observed band size: 410 kDa

IHC: ASPM Mouse mAb (Ab089860)

ASPM Mouse mAb (Ab089860) - IHC
IHC analysis of adrenal gland tissue by ASPM Mouse mAb (Ab089860).
IHC was performed using sections of the formalin-fixed paraffin-embedded adrenal gland tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with ASPM Mouse mAb (Ab089860) at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glandular cells are positively stained at the cytoplasm.

Aplicação

• Aplicação: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/400

Alvos associados (humanos)

ASPM Tbio Abnormal spindle-like microcephaly-associated protein (0 Activities)

Discover Target: ASPM

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)