ATG4D Mouse mAb - Primary antibody, specific to ATG4D, Mouse IgG1

Cat. No.: Ab090017

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Mouse

Reactive species

Human, Mouse

Isotype

Mouse IgG1

Clone Number

P2S15

Conjugation

Unconjugated

Visão geral

Mouse anti Human ATG4D Antibody, Monoclonal (P2S15), could be used for WB, IF,ICC and so on.

Specifications

Product Name

ATG4D Mouse mAb - Primary antibody, specific to ATG4D, Mouse IgG1

Sinónimos

APG4 autophagy 4 homolog D antibody | APG4 Dantibody | APG4, S. cerevisiae, homolog of, D antibody | APG4D antibody | ATG4 autophagy related 4 homolog D (S. cerevisiae) antibody | Atg4d antibody | ATG4D_HUMAN antibody | Atg4dl antibody | 9830134P12Rik ant

Especificações e pureza

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Mecanismos bioquímicos e fisiológicos

Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a reveal

Espécie hospedeira

Mouse

Especificidade

ATG4D

Imunogénio

Recombinant fusion protein of human ATG4D.

Controlo positivo

WB: A431, A549, HeLa and NIH/3T3 cell lysates. IF/ICC: U251 cells.

Conjugação

Unconjugated

Grau

Carrier Free, ExactAb™, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein G purified

Concentração

0.5 mg/mL

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: ATG4D Mouse mAb (Ab090017)

ATG4D Mouse mAb (Ab090017) - Western Blot
All lanes: ATG4D Mouse mAb (Ab090017) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 45 kDa
Exposure time: 100.0 s

IF/ICC: ATG4D Mouse mAb (Ab090017)

ATG4D Mouse mAb (Ab090017) - IF/ICC
Immunofluorescent analysis of ATG4D staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ATG4D Mouse mAb (Ab090017) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Aplicação

• Aplicação: Dilution info
• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/10 - 1/50 ;

Alvos associados (humanos)

ATG4D Tbio Cysteine protease ATG4D (0 Activities)

Discover Target: ATG4D

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)