beta 2 Microglobulin Mouse mAb - Primary antibody, specific to beta 2 Microglobulin, IgG2a

Cat. No.: Ab169199

GRAU & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, 1.0 mg/mL

Application

IF/ICC, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

CD01/2B7

Conjugation

Unconjugated

Visão geral

Application
WB: 1/1,000 - 1/10,000; Predicted molecular weight: 13.7 KDa
ICC/IF: 1/200

Especificações

Product Name

beta 2 Microglobulin Mouse mAb - Primary antibody, specific to beta 2 Microglobulin, IgG2a

Sinónimos

B2M | B2MG_HUMAN | Beta 2 microglobin | Beta 2 microglobulin | Beta 2 microglobulin precursor | Beta chain of mhc class 1 proteins | Beta chain of MHC class I molecules | Beta-2-microglobulin form pI 5.3 | CDABP0092 | Hdcma22p | IMD43 | beta-2-microglobul

Especificações e pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,1.0 mg/mL

Espécie hospedeira

Mouse

Especificidade

beta 2 Microglobulin

Imunogénio

Recombinant protein corresponding to Human beta-2-microglobulin

Controlo positivo

WB: HeLa, Raji, THP-1 and HepG2 Whole cell Lysates ICC/IF: HeLa cells

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isótipo

IgG2a

Tipo de corrente de luz

kappa

SDS-PAGE

150.0 KDa

Método de purificação

Protein A purified

Concentração

1.0 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imagens

Western Blot: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

Western Blot: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

Western Blot: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

Western Blot: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - Western Blot
All lanes: beta 2 Microglobulin Mouse Antibody (Ab169199) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 13.7 kDa
Observed band size: 13.8 kDa
Exposure time: 43.0 s 

ICC/IF: beta 2 Microglobulin Mouse mAb (Ab169199)

beta 2 Microglobulin Mouse mAb (Ab169199) - ICC/IF
IF analysis of beta 2 Microglobulin (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta 2 Microglobulin Mouse mAb (Ab169199) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/500 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Aplicação

Aplicação

Dilution info

IF/ICC

1/200

WB

1/1,000 - 1/10,000; Predicted molecular weight: 13.7 KDa

Alvos associados (humanos)

B2M Tbio Beta-2-microglobulin (0 Activities)

Discover Target: B2M

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (beta 2 Microglobulin Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.