beta Actin Mouse mAb - Primary antibody, for WB,Flow,ICC/IF,ELISA various applications, from Human, generated in Mouse, specific to beta actin, IgG1

Cat. No.: Ab155822

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, See COA

Application

Flow Cytometry, IF/ICC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

FG01/2D10

Conjugation

Unconjugated

Visão geral

Application：
WB: 1/1,000-1/20,000; Predicted molecular weight: 42 kDa.
Flow: 0.016-2.0μg/mL
ICC/IF: 0.5μg/mL
Protein Function:
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Specifications

Product Name

beta Actin Mouse mAb - Primary antibody, for WB,Flow,ICC/IF,ELISA various applications, from Human, generated in Mouse, specific to beta actin, IgG1

Sinónimos

A26C1A antibody | A26C1B antibody | ACTB antibody | ACTB_HUMAN antibody | Actin beta antibody | Actin cytoplasmic 1 antibody | Actin, cytoplasmic 1, N-terminally processed antibody | Actx antibody | b actin antibody | b-actin antibody | Beta cytoskeletal

Especificações e pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA

Espécies hospedeiras

Mouse

Espécie hospedeira

Mouse

Especificidade

beta actin

Aplicação

WB,Flow,ICC/IF,ELISA

Imunogénio

Synthetic peptide in beta Actin

Controlo positivo

WB: A549, MCF-7, HeLa, Hep G2, Raji, Ramos, MOLT-4, Jurkat, LNCap, A431, HEK293, NIH/3T3, K562, HT-29, THP-1 lysates. ICC/IF: HeLa cells. Flow: HeLa cells.

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Tipo de anticorpo

Primary antibody

Clonalidade

Monoclonal antibodies

Isótipo

IgG1

Tipo de corrente de luz

kappa

Método de purificação

Protein A purified

Fonte

Hybridoma supernatant

Concentração

See COA

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: beta Actin Mouse mAb (Ab155822)

beta Actin Mouse mAb (Ab155822) - Western Blot
All lanes: beta Actin Mouse mAb (Ab155822) at 1/10000 dilution

Samples: Lysates at 20 µg per lane

Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/10000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

ICC/IF: beta Actin Mouse mAb (Ab155822)

beta Actin Mouse mAb (Ab155822) - ICC/IF
IF analysis of beta Actin (green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (AF488) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Flow Cytometry: beta Actin Mouse mAb (Ab155822)

beta Actin Mouse mAb (Ab155822) - Flow Cytometry
Flow analysis of beta Actin (red line) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes. Cells were stained with beta Actin Mouse mAb (Ab155822) at 1/2000 dilution for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark . Unlabeled sample (black line) was used as a negative control.

Aplicação

• Aplicação: Dilution info
• IF/ICC:

  0.5 μg/mL ;

• Flow Cytometry:

  0.016-2.0μg/mL ;

• WB:

  1/1,000-1/20,000 ;

Alvos associados (humanos)

ACTB Tbio Actin, cytoplasmic 1 (0 Activities)

Discover Target: ACTB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (beta Actin Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.