Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interactio
Espécie hospedeira
Rabbit
Especificidade
CBLN1
Imunogénio
A synthetic peptide derived from human CBLN1
Controlo positivo
WB: HUVEC, A172, RAW264.7, A549 lysates. IHC: Human tonsil, esophagus cancer tissues.
Conjugação
Unconjugated
Isótipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificação
Antigen affinity purified
Concentração
See COA
Product Line
biological_products
Armazenamento e envio
Forma
Liquid
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Aplicação
Aplicação
Dilution info
IHC
1/50-1/200
WB
1/1000 - 1/5000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Revisões
Avaliações dos Clientes
Application Protocols
Validated applications, suggested dilutions, and positive control tissues/cell lines: Not specified for this item; refer to CoA/Spec Sheet.
General starting points (literature, non-item-specific):
WB: Start 1:500–1:2,000 for primary; optimize based on signal-to-noise. Positive control: cerebellum lysate.
IHC/IF: Start 1:100–1:500; optimize fixation (PFA) and antigen retrieval (heat-mediated citrate or Tris-EDTA) depending on epitope.
ELISA: Start 0.1–1 µg/mL for detection; adjust based on standard curve and background.
IP: 1–5 µg antibody per mg lysate protein as a starting point; optimize for binding and wash conditions.
These are general guidelines only and not item-specific recommendations.
Biological Roles
General background on CBLN1 (cerebellin-1; literature):
CBLN1 is a secreted glycoprotein of the C1q/TNF superfamily predominantly expressed in cerebellar granule cells. It functions as a synaptic organizer bridging presynaptic neurexins (NRXN) and postsynaptic delta-type glutamate receptors (GRID1/GRID2), promoting synaptogenesis and synaptic integrity.
Structure: CBLN1 forms homomeric hexamers (trimers of dimers) mediated by its C1q domain, enabling multivalent interactions at parallel fiber–Purkinje cell synapses.
Biology: CBLN1 plays roles in synapse formation, maintenance, and plasticity in cerebellar circuits and may have functions in other brain regions. Genetic studies in model organisms show that Cbln1 deficiency disrupts parallel fiber–Purkinje cell synapses and impairs synaptic transmission (literature).
Post-translational processing: Synthesized as a preproprotein that undergoes signal peptide cleavage and secretion; may be N-glycosylated.
Utility of anti-CBLN1 antibodies:
Enables detection and quantification of endogenous CBLN1 in tissues and cultured neurons.
Supports mapping of synaptic architecture via co-localization with NRXN/GRID markers.
Note: Host species, clonality, and epitope details for this SKU are not specified; consult the CoA/Spec Sheet for item-specific characteristics.
Buffer Applications
Not typically applicable as a standalone topic for an antibody product. Antibodies are used within standard immunoassay buffers rather than serving as buffering agents.
General buffer guidance (literature):
Storage/assay buffers: PBS or TBS at pH 7.2–7.6; optional additives include 0.05% Tween-20 for washing and 1–5% BSA or non-fat milk for blocking (WB/IHC/ELISA). Exact formulation for this item is not specified; refer to the CoA.
Avoid chelators or high detergent levels when they could disrupt protein–protein interactions (e.g., for IP/Co-IP), unless required by the protocol.
Green Alternatives
Not directly applicable. Antibody usage is aqueous and does not typically involve organic solvent selection where greener replacements would be considered.
General sustainability practices for antibody workflows (literature):
Minimize single-use plastics by employing reusable buffer reservoirs where compatible with sterility requirements.
Prepare buffers in bulk and filter-sterilize to reduce packaging waste.
Optimize antibody titers to reduce reagent consumption while maintaining signal-to-noise.
Use non-toxic preservatives and detergents where feasible, and dispose of azide-containing wastes according to institutional and environmental regulations.
Pharmaceutical Uses
Not applicable. This product is supplied for research use only and is not intended for human or veterinary use, diagnostic procedures, or as a therapeutic.
General note (literature): In biopharmaceutical manufacturing, antibodies may serve as analytical reagents for process monitoring; however, no such application is specified for this item. Always adhere to the Research Use Only designation.
Physical Properties
Item-specific physical properties: Not specified for this item; refer to CoA/Spec Sheet.
General guidance for protein antibodies (literature):
Apparent molecular mass: Intact IgG ~150 kDa; Fab ~50 kDa; scFv ~25–30 kDa. Exact value depends on format/isotype and is not specified here.
Appearance: Antibody solutions are typically clear to slightly opalescent; lyophilized forms are typically white to off-white solids. Not specified for this item.
Solubility: Antibodies are water-soluble in physiological buffers (e.g., PBS, TBS) with stabilizers (e.g., 0.02–0.05% sodium azide or ProClin, 10–50% glycerol) depending on formulation (not specified for this item).
pI: IgG pI commonly 6–9 depending on isotype/clone (literature). Not item-specific.
Stability considerations: Antibodies are sensitive to repeated freeze–thaw; storage at recommended temperatures and in stabilizing buffers preserves binding activity.
UV absorbance: Proteins typically quantified at A280 using extinction coefficients specific to sequence; extinction coefficient for this antibody is not specified.
Quality and Grades
Item-specific grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
General antibody quality considerations (literature):
Validation: Antibody quality is typically supported by application validation (e.g., WB, IHC, IF, ELISA), antigen affinity measurements, and species cross-reactivity profiling. Validated applications for this item are not specified.
Specificity controls: Pre-adsorption with immunogen peptide (for peptide-raised polyclonals), knockout/knockdown controls, or orthogonal validation (mass spectrometry) are recommended to confirm target specificity.
Purification: Common methods include Protein A/G affinity, antigen affinity, and IgG fractionation. The purification method for this item is not specified.
Formulation: Buffers often include PBS/TBS, pH 7–8, with stabilizers (e.g., glycerol) and preservatives (e.g., sodium azide). Exact components and concentrations for this item are not specified.
Batch documentation: Certificate of Analysis (CoA) typically provides lot-specific concentration (mg/mL), recommended dilutions, and QC data (SDS-PAGE purity, endotoxin as applicable). Please refer to the CoA/Spec Sheet for this item.
Reaction and Applications
Classical synthetic organic reactions are not applicable to antibody reagents.
General research applications for anti-CBLN1 antibodies (literature, non-item-specific):
Immunoblotting (WB) to detect CBLN1 expression and isoform sizes in tissue lysates (e.g., cerebellum) under reducing conditions.
Immunohistochemistry (IHC/IHC-P/IHC-Fr) to localize CBLN1 in neural tissues, particularly cerebellar granule cells and synaptic layers.
Immunofluorescence (IF) for subcellular localization and co-localization with synaptic markers.
ELISA or sandwich-ELISA for quantitative measurement of CBLN1 in biological samples using matched capture/detection pairs.
Immunoprecipitation (IP/Co-IP) to study CBLN1 interaction partners.
Note: Specific validated applications, working dilutions, and positive control recommendations for this SKU are not specified; refer to the CoA/Spec Sheet.
Reaction Conditions
Not applicable to chemical reactions. For immunoassay workflows, conditions depend on application and antibody properties.
General conditions (literature, non-item-specific):
WB: Denaturing SDS-PAGE; transfer to PVDF/nitrocellulose; block (e.g., 5% milk in TBST); primary incubation 1–16 h at 4–25°C; appropriate HRP- or fluorophore-conjugated secondary.
IHC/IF: Antigen retrieval and permeabilization as needed; block with serum/protein; primary 1–16 h at 4°C; use species-matched secondary antibodies; include no-primary controls.
ELISA: Coat capture at 1–10 µg/mL (for capture antibodies), block, add sample, detect with biotinylated/HRP secondaries; develop with TMB and read at 450 nm.
Note: Recommended dilutions, incubation times, and retrieval methods for this SKU are not specified; refer to the CoA/Spec Sheet.
Safety and Handling
GHS classification, signal word, pictograms, and H-statements: Not specified for this item; refer to SDS.
General laboratory safety (literature/best practice):
Handle antibody preparations as laboratory reagents for research use only. Avoid ingestion, inhalation of aerosols, and contact with skin/eyes. Use standard PPE: lab coat, nitrile gloves, safety glasses.
Work in a clean area to prevent microbial contamination of protein solutions. If the formulation includes preservatives (e.g., sodium azide or ProClin), avoid contact and follow proper waste disposal protocols. Composition of preservatives is not specified; consult the CoA/SDS.
Incompatibilities: Do not mix with strong oxidizers or reactive chemicals that can denature proteins. Avoid metal-catalyzed oxidation and extreme pH or temperature.
Special risks: Repeated freeze–thaw cycles can cause aggregation and loss of binding activity—aliquot upon first thaw. Avoid vigorous vortexing or foaming.
First aid (general): In case of skin/eye contact, rinse with water. If inhaled, move to fresh air. If swallowed, rinse mouth. Seek medical advice if irritation persists. Always defer to the SDS for authoritative safety and first-aid instructions.
Spill response: Wipe with absorbent material; decontaminate surfaces with appropriate lab disinfectant (e.g., 70% ethanol). Dispose of waste per institutional guidelines.
Solvent Selection
Not typically applicable. Antibodies are aqueous biomolecules that are stored and used in biological buffers rather than organic solvents.
General guidance (literature):
Preferred media: PBS or TBS, often with 0.05% Tween-20 for washing steps in immunoassays; 10–50% glycerol may be included for freeze–thaw stability at −20°C.
Avoid: Strong organic solvents, extreme pH (<5 or >9), and high denaturant concentrations unless performing deliberate denaturation (e.g., for epitope mapping).
Storage and Reconstitution
Storage conditions (item-specific): Store at −20°C. Avoid repeated freezing and thawing (Product Data). Shipped in ice chest with ice pads.
Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. If lyophilized, follow the lot-specific instructions for reconstitution buffer, volume, and final concentration.
General best practices (literature):
Upon receipt, briefly centrifuge vials to collect contents. If the product is liquid, aliquot into low-protein-binding tubes to avoid freeze–thaw cycles. For −20°C storage, inclusion of 30–50% glycerol can permit storage without freezing (if compatible with formulation; verify in CoA).
Avoid foaming and vigorous agitation; mix by gentle inversion.
Thaw on ice and keep on ice during use. Do not heat above room temperature unless protocol requires it.
Record freeze–thaw history for each aliquot. Discard aliquots showing precipitation or loss of performance.
For exact buffer composition, concentration (mg/mL), preservative content, and stability data, consult the lot-specific CoA/Spec Sheet.
Structure and Identity
Item-specific identifiers (CAS, CID, InChIKey, SMILES, molecular formula/MW): Not specified for this item; refer to CoA/Spec Sheet.
General information (literature):
The product is an antibody directed against the protein target CBLN1 (cerebellin-1). Antibodies are immunoglobulins composed of two heavy and two light polypeptide chains forming a Y-shaped quaternary structure stabilized by disulfide bonds.
Common research formats include polyclonal IgG, monoclonal IgG (e.g., mouse or rabbit), and recombinant monoclonal antibodies; isotype and clonality are critical for selecting appropriate secondary reagents but are not specified for this item.
Typical functional groups and motifs in antibodies include multiple disulfide linkages, N-linked glycosylation sites (Fc region, isotype-dependent), and variable domains (VH/VL) conferring antigen specificity. The Fc region mediates effector functions and influences species cross-reactivity of secondary antibodies.
Antibody preparations may be supplied as liquid in stabilizing buffer or lyophilized; formulation (buffer, pH, preservatives such as sodium azide, carrier proteins like BSA or gelatin) is not specified for this item; refer to CoA/Spec Sheet.
Synthetic Utility
Not applicable. Antibodies are not small-molecule building blocks and are not used in classical organic synthesis.
General utility in experimental workflows (literature):
As affinity reagents, antibodies can be immobilized on resins or beads for immunoaffinity purification of CBLN1 or associated complexes.
In proteomics, antibodies can enrich targets prior to LC–MS/MS analysis.
Item-specific resin coupling chemistry or affinity matrices are not specified for this SKU; consult the CoA/Spec Sheet if applicable.
Target Specificity
Item-specific details are not provided. Refer to CoA/Spec Sheet.
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