CD14 Mouse mAb (Biotin) - Primary antibody, specific to CD14

Cat. No.: Ab210089

GRAU & PUREZA ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

UCHM-1

Conjugation

Biotin

Visão geral

Mouse anti Human CD14 Antibody, Monoclonal (UCHM-1), could be used for Flow, WB, IHC, IF, ICC, IP and so on.

Especificações

Product Name

CD14 Mouse mAb (Biotin) - Primary antibody, specific to CD14

Sinónimos

CD14 antigen | CD14 molecule | CD14 | monocyte differentiation antigen CD14 | Myeloid cell-specific leucine-rich glycoprotein | CD 14 antibody | CD_antigen=CD14 antibody | CD14 antibody | CD14 antigen antibody | CD14 molecule antibody | CD14_HUMAN antibod

Especificações e pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos e fisiológicos

Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molec

Espécie hospedeira

Mouse

Especificidade

CD14

Imunogénio

Human Thymocytes followed by peripheral blood mononuclear cells

Controlo positivo

Flow: Human peripheral blood monocytes

Conjugação

Biotin

Grau

ExactAb™, Validated

Tipo de corrente de luz

kappa

Método de purificação

Protein G purified

Concentração

0.5 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at 2-8°C,Protected from light,Do not freeze

Enviado em

Wet ice,Do not freeze

Estabilidade e armazenamento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imagens

Flow Cytometry: CD14 Mouse mAb (Biotin) (Ab210089)

CD14 Mouse mAb (Biotin) (Ab210089) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood (right panel) compared to an isotype control (left panel). Cells were stained with CD14 Mouse mAb (Biotin) (Ab210089) at 0.2μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).

Flow Cytometry: CD14 Mouse mAb (Biotin) (Ab210089)

CD14 Mouse mAb (Biotin) (Ab210089) - Flow Cytometry
Flow cytometry analysis of CD14 expression by human peripheral blood monocytes (red) compared to an isotype control (blue). Cells were stained with CD14 Mouse mAb (Biotin) (Ab210089) at 0.2μg/test for 1 hour at 4°C. Blue - Mouse IgG (Biotin) (Ab180306). Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Black - Unlabelled control, cells without incubation with primary antibody.

Aplicação

Aplicação

Dilution info

Flow Cytometry

≤0.3 μg/test, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume

Alvos associados (humanos)

CD14 Tbio Monocyte differentiation antigen CD14 (0 Activities)

Discover Target: CD14

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD14 Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.