CD22 Mouse mAb - Primary antibody, specific to CD22, Mouse IgG1

Cat. No.: Ab094278

GRAU & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Depletion, Flow Cytometry, Functional Assay, IP

Host species

Mouse

Reactive species

Mouse

Isotype

Mouse IgG1

Clone Number

Cy34.1

Conjugation

Unconjugated

Visão geral

Mouse anti Mouse CD22 Antibody, Monoclonal (Cy34.1), could be used for in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κLight Chain (clone MAR 18.5), Flow, IP and so on.
Application:
in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κLight Chain (clone MAR 18.5): Use at an assay dependent concentration. 
Flow: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration. 
Protein Function
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Especificações

Product Name

CD22 Mouse mAb - Primary antibody, specific to CD22, Mouse IgG1

Sinónimos

Lyb8|Lyb-8|B-cell receptor CD22|BL-CAM|B-lymphocyte cell adhesion molecule|CD22 antigenMGC130020|CD22 molecule|CD22|sialic acid binding Ig-like lectin 2|Sialic acid-binding Ig-like lectin 2|Siglec2|Siglec-2|SIGLEC2FLJ22814|T-cell surface antigen Leu-14|B cell receptor CD22 precursor antibody|B lymphocyte cell adhesion molecule antibody|B-cell receptor CD22 antibody|B-lymphocyte cell adhesion molecule antibody|BL CAM antibody|BL-CAM antibody|BLCAM antibody|CD 22 antibody|CD22 antibody|CD22 antigen antibody|CD22 molecule antibody|CD22 protein antibody|Lectin 2 antibody|Leu14 antibody|Lyb8 antibody|MGC130020 antibody|sialic acid binding Ig like lectin 2 antibody|Sialic acid binding immunoglobulin like lectin 2 antibody|Sialic acid-binding Ig-like lectin 2 antibody|SIGLEC 2 antibody|Siglec-2 antibody|SIGLEC2 antibody|T cell surface antigen Leu 14 antibody|T-cell surface antigen Leu-14 antibody

Especificações e pureza

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Espécie hospedeira

Mouse

Especificidade

CD22

Imunogénio

B10.D2 mouse splenocytes

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

SDS-PAGE

150 kDa

Método de purificação

Protein G purified

Concentração

See COA

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at 2-8°C,Do not freeze

Enviado em

Wet ice,Do not freeze

Estabilidade e armazenamento

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Imagens

Flow Cytometry: CD22 Mouse mAb (Ab094278)

CD22 Mouse mAb (Ab094278) - Flow Cytometry
Two-color flow cytometric analysis of CD22 expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Mouse IgG Isotype Control Antibody (Biotin) (Ab180306; Left panel) or CD22 Mouse mAb (Ab094278; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD22 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Aplicação

Aplicação

Dilution info

IP

Use at an assay dependent concentration.

Flow Cytometry

Use at an assay dependent concentration.

in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κLight Chain (clone MAR 18.5)

Use at an assay dependent concentration.