GRAU & PUREZACarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
Validated, Carrier Free, ExactAb™, 1.0 mg/mL Carrier Free,ExactAb™,Validated para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.
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Armazenamento e envio
Store at -20°C,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.
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Documentos de qualidade
SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.
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Comprovação documental
Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.
Visão geral
Rabbit anti Human Cdc20 Antibody, Polyclonal, could be used for WB, IHC and so on. Application WB: 1/500-1/1000 IHC: 1/50-1/100 Protein Function Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates.
Especificações
Product Name
Cdc20 Antibody - Primary antibody, specific to Cdc20, Rabbit IgG
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CDC20.
Controlo positivo
WB: A431, THP-1, U-2 OS and C6 cell lysates. IHC: Rat brain tissue.
Conjugação
Unconjugated
Grau
Carrier Free, ExactAb™, Validated
Isótipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificação
Immunogen affinity purified
Concentração
1.0 mg/mL
Product Line
Produtos biológicos
Armazenamento e envio
Forma
Liquid
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imagens
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 20.0 s
Cdc20 Antibody (Ab095575) - Western Blot All lanes: Cdc20 Antibody (Ab095575) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 54 kDa Observed band size: 50 kDa Exposure time: 10.0 s
Cdc20 Antibody (Ab095575) - IHC Immunohistochemical analysis of CDC20 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the Cdc20 Antibody (Ab095575) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Aplicação
Aplicação
Dilution info
IHC
1/50 - 1/100
WB
1/500-1/1000
Documentação
📋 Ficha de dados de segurança (FDS)
Documento abrangente sobre perigos, manuseamento, armazenamento e conformidade regulamentar.
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