COX IV Mouse mAb - Primary antibody, specific to COX4I1, Mouse IgG1

Cat. No.: Ab097414

GRADE & PURITY Validated, ExactAb™, 2.0 mg/mL

Application

Flow Cytometry, IF/ICC, IHC, IP, WB

Host species

Mouse

Reactive species

Goat, Hamster, Human, Monkey, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

4D11-B3-E8

Conjugation

Unconjugated

Visão geral

Mouse anti Human COX IV Antibody, Monoclonal antibody(4D11-B3-E8), could be used for WB, IHC, ICC, IF, Flow, IP and so on.

Application:

WB: 1/1000 

IHC: 1/50-1/200

ICC/IF: 1/100-1/200 

Flow: 1/100 

IP: 1/20 

Protein Function

This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Specifications

Product Name

COX IV Mouse mAb - Primary antibody, specific to COX4I1, Mouse IgG1

Sinónimos

AL024441 antibody | COX 4 antibody | COX IV 1 antibody | COX IV antibody | COX IV-1 antibody | Cox4 antibody | COX41_HUMAN antibody | Cox4a antibody | COX4B antibody | COX4I1 antibody | COX4I2 antibody | COX4L2 antibody | COXIV antibody | Cytochrome c oxi

Especificações e pureza

ExactAb™, Validated, 2.0 mg/mL

Espécie hospedeira

Mouse

Especificidade

COX4I1

Imunogénio

A synthetic peptide derived from human COX IV (AA 1-169).

Controlo positivo

WB: Jurkat, NIH/3T3, HeLa, C6, K562, MCF7, THP-1, Raji; IHC: Human colorectal carcinoma; ICC/IF: HeLa cells; IP: HeLa.

Conjugação

Unconjugated

Grau

ExactAb™, Validated

Isótipo

Mouse IgG1

SDS-PAGE

150 kDa

Método de purificação

Protein G purified

Concentração

2.0 mg/mL

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - Western Blot
All lanes: COX IV Mouse mAb (Ab097414) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 5.0 s

ICC/IF: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - ICC/IF
Immunocytochemistry analysis of COX IV (green) in HeLa cells using COX IV Mouse mAb (Ab097414).

IHC: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - IHC
Immunohistochemistry analysis of paraffin-embedded Human colorectal carcinoma using COX IV Mouse mAb (Ab097414). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IP: COX IV Mouse mAb (Ab097414)

COX IV Mouse mAb (Ab097414) - IP
Immunoprecipitation analysis of COX IV (4D11) in HeLa cell lysates using COX IV Mouse mAb (Ab097414)

Aplicação

• Aplicação: Dilution info
• IP:

  1/20;

• WB:

  1/1000 ;

• IHC:

  1/50-1/200;

• IF/ICC:

  1/100-1/200;

• Flow Cytometry:

  1/100;

Alvos associados (humanos)

COX4I1 Tbio Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (0 Activities)

Discover Target: COX4I1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (COX IV Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.