Carrier Free,Low Endotoxin,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE&HPLC),See COA Azide Free,Carrier Free,Low Endotoxin,PBS Only,Validated para fluxos de trabalho cromatográficos e analíticos sensíveis que exigem interferência mínima da linha de base.
Store at -20°C,Avoid repeated freezing and thawing Envio Ice chest + Ice pads. Consulte o COA específico do lote para obter as especificações exatas.
SDS, COA, ficha técnica e folha de especificações disponíveis para download. COA específico do lote acessível por meio de pesquisa por número de lote.
Citado em 0 publicações revisadas por pares nas áreas de cromatografia, síntese orgânica e reações de acoplamento cruzado.
Mouse anti Human EGFR Antibody, Monoclonal (EGFR.1), could be used for WB, IHC, ICC/IF, IP, Flow, Neutralization, IP-Mass Spectrometry and so on.
Application
ICC/IF: 5 µg/mL
IP: 2 µg/mL
Flow: 1-5 ug/mL
IHC: Use at an assay dependent concentration.
WB: Use at an assay dependent concentration.
Neutralization: Use at an assay dependent concentration.
IP-Mass Spectrometry: Use at an assay dependent concentration.
Protein Function
Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin.
Isoform 2 may act as an antagonist of EGF action.
EGFR Mouse mAb (Ab101156) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling EGFR (red) with EGFR Mouse mAb (Ab101156) at 0.5 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.
5.0 μg/mL
Use at an assay dependent concentration.
Use at an assay dependent concentration.
2 µg/mL
1-5 μg/mL
Use at an assay dependent concentration.
Use at an assay dependent concentration.
Documento abrangente sobre perigos, manuseamento, armazenamento e conformidade regulamentar.
<<<<<<< HEAD Baixar SDS → ======= Baixar SDS → >>>>>>> release248-p3Dados de qualidade específicos do lote. Introduza o número de lote para obter o COA exato.
Consultar COA →Resumo de referência rápida das especificações e aplicações do produto.
Ver ficha técnica →Atributos de qualidade completos e critérios de aceitação para este grau.
Ver folha de especificações →| Activity Type | Activity Value -log(M) | Mechanism of Action | Activity Reference | Publications (PubMed IDs) |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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| Activity Type | Relation | Activity value | Units | Action Type | Journal | PubMed Id | doi | Assay Aladdin ID |
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Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Data | Item |
|---|---|---|---|
 ZJ24F0707957 | Certificate of Analysis | Oct 08, 2026 | Ab101156 |
| ZJ24F0707958 | Certificate of Analysis | Oct 08, 2026 | Ab101156 |
| ZJ24F0707959 | Certificate of Analysis | Oct 08, 2026 | Ab101156 |
O nosso guia de seleção de graus abrange pureza, estado do estabilizador e adequação à aplicação para todas as variantes do nosso catálogo.
Ver o guia de graus Carrier Free → Ver o guia de graus Validated → Ver o guia de graus Low Endotoxin → Ver o guia de graus Azide Free → Ver o guia de graus PBS Only →
