GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.See COA
Carrier Free, ExactAb™, Azide Free, Validated, See COA Azide Free,Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Visão geral
Rabbit anti Human FAK Antibody, Polyclonal, could be used for WB, IHC and so on.
Application
WB: 0.5-2 µg/mL
IHC: 5-20 µg/mL
IF/ICC: 3.25 µg/mL
Protein Function
Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
Specifications
Product Name
FAK Antibody - Primary antibody, specific to FAK, Rabbit IgG
Carrier Free, ExactAb™, Azide Free, Validated, See COA
Espécie hospedeira
Rabbit
Especificidade
FAK
Imunogénio
Recombinant FAK expressed in E.coli (AA 798-1041)
Controlo positivo
WB: C6, NIH/3T3, K562, HUVEC and MCF7 cell lysates. IHC: Human Liver tissue, Human Thyroid tissue, Human Liver cancer tissue. IF/ICC: HeLa cells.
Conjugação
Unconjugated
Grau
Azide Free, Carrier Free, ExactAb™, Validated
Isótipo
Rabbit IgG
SDS-PAGE
150 kDa
Método de purificação
Antigen affinity purified and Protein A purified
Concentração
See COA
Product Line
biological_products
Armazenamento e envio
Forma
Liquid
Condições de armazenamento de armazenamento
Store at -20°C,Avoid repeated freezing and thawing
Enviado em
Ice chest + Ice pads
Estabilidade e armazenamento
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imagens
FAK Antibody (Ab102794) - Western Blot All lanes: FAK Antibody (Ab102794) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 125 kDa Observed band size: 111 kDa Exposure time: 15.6 s
FAK Antibody (Ab102794) - Western Blot All lanes: FAK Antibody (Ab102794) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 125 kDa Observed band size: 111 kDa Exposure time: 15.6 s
FAK Antibody (Ab102794) - IHC DAB staining on IHC Samples: Human Liver Tissue Primary Ab: 20 µg/mL FAK Antibody (Ab102794) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
FAK Antibody (Ab102794) - IHC DAB staining on IHC Samples: Human Thyroid Tissue Primary Ab: 20 µg/mL FAK Antibody (Ab102794) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
FAK Antibody (Ab102794) - IHC DAB staining on IHC Samples: Human Liver cancer Tissue Primary Ab: 20 µg/mL FAK Antibody (Ab102794) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.
FAK Antibody (Ab102794) - IF/ICC
IF analysis of FAK Antibody (Ab102794) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with FAK Antibody (Ab102794) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (D1372407) (blue).
Aplicação
Aplicação
Dilution info
IHC
5-20 µg/mL
WB
0.5-2 µg/mL
IF/ICC
3.25 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Revisões
Avaliações dos Clientes
Perguntas frequentes
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (FAK Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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