Extracellular system for translating viral mRNA
Translation of poliovirus and hepatitis C virus mRNA with mammalian cell S10 extracts.
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RNA standard transcription reaction
The 3'-5' exonuclease activity of the large fragment of DNA polymerase I (Klenow enzyme) was utilized to convert the 3' sticky ends into flat ends. The method i
PAT method for analyzing mRNA Poly(A) tail length assay
The PAT method [ PCR poly(A) test ] can quickly and sensitively analyze specific mRNA from total RNA and quantitatively estimate the length of RNA poly(A) tails
Fe(II)-EDTA protection assay experiment
Fe(II)-EDTA is capable of catalyzing the breakage of RNA or DNA and is a complementary method to RNase protection analysis. It has the advantage that there is l
RNA bulk transcription synthesis
The typical recovery rate of the RNA standard transcription system is lugRNA/ug plasmid DNA, which can be increased to 5~l0ug RNA/ug plasmid DNA by using the fo
Hepatitis A virus RNA extraction
RNA Laboratory Techniques Manual (Molecular Cloning - Laboratory Guide Series)
Recovery of RNA fragments from polyacrylamide gels
The best method for recovering RNA fragments from poly(lactonamide) gels is the crush-and-soak method, which provides excellent recovery of high-purity single-s
RNA in situ hybridization assay
In situ hybridization (ISH), also known as hybrid histochemistry or hybridization for cytology, is a technique that enables morphological demonstration of the p
Photo-activated nucleotide analog-mediated RNA-protein cross-linking experiments
Photochemical cross-linking technology is an effective means to study the interaction between RNA and protein in nuclear enameled egg complexes. The basic princ