Yeast precursor mRNA splicing extract assay
The splicing of eukaryotic pre-mRNA is a two-step process, in which an intron is cut from the precursor and two exons are joined together to form the mature mRN
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Natural RNA library screening and protein-specific binding RNA sequence experiments
In cells, protein-RNA binding is involved in a variety of physiological regulation and developmental processes. Direct protein-RNA interactions occur during mRN
Analysis of mRNA degradation experiments by cell-free extracts of higher eukaryotes
Changes in the environment of the cell, the state of the replication cycle, and the state of differentiation can cause changes in the half-life of some mRNAs. T
Preparation of nuclear and cytoplasmic S100 extracts of HeLa cells for in vitro splicing reaction assay
Cytosolic extracts can be used for splicing of precursor mRNAs. The S100 extract, although it includes many splicing factors, does not have splicing activity be
Preparation of yeast total RNA
Good quality total RNA can be extracted from yeast cells.
Preparation of yeast tRNA by phenol method
By purification on DEAE cellulose columns, small amounts of DNA, large molecular RNA, proteins, polysaccharides and other impurities were removed, and finally a
Preparation of fungal total RNA
Total RNA was extracted from a variety of different fungi.
Purification of splicing factor SR protein assay
Precursor mRNA ( pre-mRNA ) splicing factors The SR (serine/argininc rich) family of proteins is an important player in the assembly of splices onto precursor m
RNaseH cleavage protection experiments for oligonucleotide targeting in RNA- protein complexes
Oligonucleotide-targeted RNase II digestion assay is a method to analyze RNA protein complexes (RNP, ribonucleoprotein particles), both for crude extracts and p