Construction of recombinant DNA by polymerase chain reaction
The polymerase chain reaction constructs recombinant DNA for a wide range of uses (1) for sequencing primers (2) targeted mutagenesis (3) nucleic acid hybridiza
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Steps for SSR labeling experiments
SSR simple sequence repeat markers can be used to: design pairs of primers with a specific sequence of microsatellite regions, and polymorphism of SSR loci amon
Gene transfection (calcium phosphate-DNA co-precipitation)
Gene transfection technology can be applied to: genome function research (gene expression regulation, gene function, signal transduction and drug screening rese
Determination of DNA by chemical method - Diphenylamine colorimetric method
Chemical determination of DNA content can be used for (1) estimation of plasmid concentration prior to transformation and transfection, and (2) assay of acquire
Removal of ethidium bromide from DNA by extraction with organic solvents
To remove ethidium bromide from CsCl-gradient purified DNA, repeated extraction with organic solvents is commonly used. Subsequently, CsCl can be removed by dia
Plasmid DNA purification by polyethylene glycol precipitation assay
This method was first devised by Richaed Treisman (ICRF, London, UK) with reference to the work of Lis, who was the first to use polyethylene glycol (PEG) to is
Chemiluminescent Dideoxy DNA Sequencing Assay
Standard double deoxy DNA sequencing can be easily and efficiently detected using non-isobaric labeled chemiluminescence. In the sequencing reaction, biotin-lab
Removal of ethidium bromide from DNA by ion exchange chromatography
Ethidium bromide in DNA purified by cesium chloride gradient centrifugation can be removed by ion-exchange chromatography, followed by ethanol precipitation to
Plasmid DNA preparation by the toothpick method in small quantities
Colonies picked with a toothpick from agar medium can be used directly to prepare plasmid DNA. the resulting closed-loop DNA often has too many impurities to be