Ligation of plasmid and target DNA in low melting point agarose experiment
The most time-consuming step of the plasmid cloning operation is the purification of the desired sized fragments of exogenous and plasmid DNA by electrophoresis
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Rapid small volume extraction of total plant DNA
DNA molecule is the basic material of molecular biology research, according to different experimental purposes can be taken to different extraction methods to o
Agarose gel electrophoresis of DNA
DNA electrophoresis can be used to (1) separate DNA fragments of different sizes, (2) identify target DNA fragments, and (3) purify and recover DNA fragments.
Screening of expression libraries constructed in λ phage vectors
In typical immunoscreening experiments, libraries of λ phage expression vector constructs should be spread on plates of E. coli strains that do not conta
DNA binding protein assay
This experiment was used to detect sequence-specific DNA-binding proteins in crude extracts.
DNA preparation of salmon sperm stretcher
Using this scheme, well-sheared, high-quality salmon sperm stretcher DNA can be obtained, which can be used in transformation experiments and other experiments
Preparation of cytoplasmic S-100 components
The contents of a cell contained within the cell membrane. In eukaryotic cells it refers to the portion of the cell membrane outside the inner core that contain
Screening of bacterial colonies with X-gal and IPTG (alpha complementary)
The chromogenic substrate X-gal can be mixed with bacterial cultures, mixed with solubilized top agar and spread on selective culture plates. This experiment is
Hybridization of bacterial DNA on filter membranes
This protocol describes how to hybridize radiolabeled probes to DNA of transforming bacteria immobilized on filter membranes, and methods for recovering clones