B Identification of functional subpopulations of cells
Peripheral mature B cells can be divided into several subpopulations. Traditionally, B cells have been divided into two subpopulations, CD5+ B1 cells and CD5 B2
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Cultured dendritic cells from mouse bone marrow precursor cells
Dendritic cells (DCs) are very few in number in vivo (less than 1% of splenic adherent cells), and the time-consuming isolation of DCs from vivo and their low c
Natural killer cell clearance in mice
NK cells are usually injected intravenously; in vivo clearance can be achieved by intraperitoneal injection of antibodies against NK cells, such as PK136 monocl
Macrophage chemotaxis assay
Macrophage migration is the primary condition for its immune activity at the site of infection and injury, and macrophage chemotaxis reflects its migration abil
Induction and characterization of macrophage polarization in mice
Macrophages can undergo functional activation (polarization) of different nature into M1 and M2 subpopulations with different surface molecular and functional c
Isolation and purification of human metaphase immune cells
The metaphase immune cell population is the biological basis of maternal-fetal immune tolerance, and its composition is extremely special, mainly composed of sp
Detection of antinuclear antibodies in serum by immunofluorescence technique assay
Antinuclear antibody (ANA) is an autoantibody directed against the nuclear components of cells, with no organ or germline specificity. A positive ANA is indicat
Isolation and purification of human metaphase stromal cells
Endometrial metamorphosis is a critical step for embryo implantation and successful pregnancy. The cellular composition of the metaphase tissue is quite complex
Measurement of immune cell phagocytosis
The body has phagocytosis (phagocytosis) function of the cells called phagocytes, including monocyte macrophages and neutrophils two categories. Phagocytes phag