CFSE assay for human peripheral blood T cell expansion in vitro
CFSE detection of human peripheral blood T cell expansion in vitro refers to the use of CFSE to fluorescently label live cells, which can be used for the detect
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B Phenotypic detection of cells
There are many membrane molecules on the surface of B cells, including cell surface differentiation antigen and model immunoglobulin (smlg). At different stages
Antibody potency assay
Antibody potency assay is used to test the potency of immune serum. Currently there are three main methods used to determine antibody potency: tube agglutinatio
Monoclonal antibody identification test
The identification of monoclonal antibodies is similar to that of polyclonal antibodies, and the methods used are common; however, the identification of some of
Induction and isolation of human blood mononuclear macrophages
Macrophages (MΦ) are mature cells formed by the migration of blood monocytes into tissues, and are widely distributed in the spleen, liver, abdominal cavity, n
Chemiluminescence immunoassay experiment
Chemiluminescence (CL) refers to the phenomenon of light radiation that accompanies the process of a chemical reaction. Most chemiluminescence is the luminescen
In vivo activation of macrophages
Macrophages are important natural immune cells in vivo, and in vitro detection of their function does not fully reflect their in vivo behavior, while detection
Isolation of peripheral blood single nucleated cells
Immune cell isolation techniques are developing in the direction of more and more "fine", immune cell subpopulation and even micro-immune cell isolation and ide
Evaluation of lymphocyte-mediated cytotoxicity using flow cytometry
Cytotoxicity of lymphocytes, is the killing of target cells by the release of particles containing perforin and granzyme and/or through the interaction of Fas-F