3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
Feeder cells are necessary for embryonic-derived stem cells including embryonic cancer cells (EC), embryonic stem cells (ES), and primordial germ cells (EG) to
Most cell lines grow best at pH 7.4, with little variation between cell lines; however, some normal fibroblast cell lines grow best at pH 7.4 to 7.7. However, s
Environmental metabolomics is a branch of metabolomics that focuses on the metabolic changes of organisms in response to environmental stress. Because this appr
A modification of the standard method for RNA preparation from mammalian tissues and Drosophila that focuses on rapid lysis of Juniperus communis and homogeneou
Hematopoietic stem cell transplantation is an effective treatment for malignant hematological disorders, oncological radiotherapy and severe radiation sickness
Currently, the life cycle and biology of adenovirus (A d ) infected cells have been well characterized. In A d virus infection and transgene expression, there a
Provides some methods on how to use the miniature SIV packaging system and SIV gene transfer vectors to obtain high titer and safe lentiviral vectors developed
Vacuolar viruses (FV) are non-pathogenic retroviruses that efficiently and safely transfer genes to different types of cells in different species. These viruses
This chapter describes methods for expressing multiple proteins from a single readable frame by using 2A polypeptide-linked polycis-trans vectors. When these sm
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