Gene transfer into mammalian cells using targeted filamentous phages
Because of their genetic simplicity, phages are commonly used in vector genetic optimization approaches for therapeutic gene transfer. In addition, since phage
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3228 articles
Transfection of hippocampal neuronal cells with calcium phosphate co-precipitated plasmid DNA
The use of DNA/phosphorothioate (CAPO 4) co-precipitation to transfect cells is a widely described method. Although this co-precipitation method was proposed mo
Intrauterine microelectroporation for efficient gene transfer in mouse embryos
The introduction of genes into the embryo has been successfully achieved by a new technique of electroporation of the chicken embryo. In contrast, since the mam
Optimizing in vitro mammalian cell electrotransfection
Electrotransfection can be used for studies of gene function, promoter activity, and gene regulation; it can also be used to create transgenic or knockout embry
Generation of VSV-G-pseudotyped retroviral vectors
In order to improve the efficiency of transduction, a strategy called pseudotyping, which combines retroviral particles with other virally encoded capsid protei
Translocation of target genes with surface-modified lentiviral vectors
Two types of surface modifications have been made to lentiviral vectors in order to achieve target gene translocation: (i) taking advantage of the targeting pro
Generation of foamy virus vectors and transduction of hematopoietic stem cells
Foamy virus (F V ), or spumavirus, is a nonpathogenic retrovirus developed as an integrating viral vector. Several potential advantages of F V vectors include a
lipoplex and LPD nanoparticles for in vivo gene delivery
The core of gene therapy research lies in the development of suitable delivery systems. Although most clinical experiments currently utilize viral carrier syste
Solid lipid nanoparticles for in vitro mammalian cell transfection
Solid lipid nanoparticles (S L N ) have many technical advantages over gene carriers such as cationic liposomes and cationic polymers. However, in the absence o