High-throughput method for screening polymer transfection reagents
The development of a safe and effective gene delivery system is a prerequisite for the success of clinical gene therapy. Despite the high transfer efficiency of
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3228 articles
Transposon-mediated delivery of small interfering RNAs: the "Sleeping Beauty" transposon experiment
A method for efficient delivery of pol III-driven SHRNA expression frames using the Sleeping Beauty (SB) transposon. Advantages of this system include its ease
Cross-linked hyaluronic acid matrix and film experiments with specific plasmid DNAs
Natural polysaccharides are being extensively studied as carriers for gene delivery. The non-inflammatory and non-immunogenic nature of hyaluronic acid (H A ) i
Naked DNA transporter assay using high-pressure injection technique
Among the various viral and non-viral means commonly used for nucleic acid transport and gene expression in animal cells, the naked plasmid D N A technique has
Expression and characterization of ribozymes and short hairpin RNA in mammalian cells
Functional genomic research using R N A interference techniques (R N A i ) has been carried out extensively in recent years.R N A i is the newest and most succe
Sonopuncture: an effective technique for introducing genes into chicken embryos
The acoustic pore effect may not be applicable to the introduction of genes into tissues with lumen-forming or open structures. Acoustic pore effects on neural
Experiments on a highly sensitive gene regulatory system based on a synthetic small-molecule ligand-conducted decidual hormone receptor
The RheoSwitch system is an efficient gene regulatory system based on the ecdysone receptor, which is activated by synthetic small molecule ligands. The recepto
Cell penetrating peptide-mediated peptide nucleic acid oligomer delivery assay
Site-specific knockout and non-toxic DNA delivery technologies are particularly important in the study of protein function and as a means for future therapies.
Genetic experiments for manipulating mammalian cells by microinjection
Introduction by microinjection can be used for any type of adherent cell, including primary cells. Since siRNA for any target gene can be obtained simply and ra